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Recombinant Saccharomyces cerevisiae Alpha-factor-transporting ATPase (STE6), partial

Recombinant Saccharomyces cerevisiae Alpha-factor-transporting ATPase (STE6), partial

Code	CSB-YP319019SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP319019SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP319019SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP319019SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP319019SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

STE6

Uniprot No.

P12866

Alternative Names

STE6; YKL209C; Alpha-factor-transporting ATPase; EC 7.4.2.7; Mating factor A secretion protein STE6; Multiple drug resistance protein homolog; P-glycoprotein

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

Gene References into Functions

Ste6p follows the PURE pathway--a general pathway for the internalization of plasma membrane proteins that involves phosphorylation, ubiquitination, recognition and endocytosis PMID: 15086792
STE6 recycling is controlled by ubiquinination in the early endocytic pathway. PMID: 15800066
Hsp70/40s act before ubiquitination and facilitate Ste6p association with E3 ubiquitin ligase, suggesting an active role for chaperones. Furthermore, polyubiquitination was a prerequisite for retrotranslocation, which required the Cdc48 complex and ATP. PMID: 18191224

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

ABC transporter superfamily, Alpha-factor sex pheromone exporter (TC 3.A.1.206) family

Database Links

KEGG: sce:YKL209C

STRING: 4932.YKL209C

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