Recombinant Saccharomyces cerevisiae Cell division control protein 13 (CDC13), partial

Code CSB-YP333735SVG
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Source Yeast
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Code CSB-EP333735SVG
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Source E.coli
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Code CSB-EP333735SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333735SVG
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Source Baculovirus
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Code CSB-MP333735SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CDC13
Uniprot No.
Alternative Names
CDC13; YDL220CCell division control protein 13
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Single-stranded telomeric DNA-binding protein that regulates telomere replication. Has a role in both positive and negative regulation. Promotes [TG(1-3)] strand lengthening via interaction with EST1. Promotes [C(1-3)A] strand re-synthesis by DNA polymerase alpha via interaction with POL1. Negatively regulates telomere elongation of the G strand via binding with STN1 thereby inhibiting telomerase activity.
Gene References into Functions
  1. DNA binding by Cdc13 employs a highly cooperative interface whereby sequence diversity is accommodated through plastic binding modes. PMID: 30249661
  2. The core Cdc13 ssDNA-binding activity is wholly contained within a single tight-binding oligosaccharide/oligonucleotide/oligopeptide binding (OB)-fold. PMID: 24057216
  3. Dimerization of the OB2 domain of Cdc13 is required for proper Cdc13, Stn1, Ten1 (CST) assembly and productive telomere capping. PMID: 23177925
  4. Cdc13, and a S314D mutation negatively affected telomere length. Our findings provide new targets in Cdc13 - a key telomerase regulatory protein for modulation of telomere dynamics PMID: 23181431
  5. regulated binding of Cdc13 to chromosome termini dictates subsequent interaction of a recruitment-competent telomerase complex with telomeres PMID: 23390975
  6. show that both Cdc48 and Vms1, but not Cdc48 cofactors Ufd1 and Ufd2, are crucial for the degradation of Cdc13, a telomere regulator PMID: 22718752
  7. Cdc13 SUMOylation restrains telomerase functions by promoting Cdc13 interaction with the telomerase inhibitor Stn1 without affecting end protection. PMID: 21743457
  8. Cdc13-Pol1 interaction revealed multiple mechanisms by which dimerization regulates telomere lengths in vivo. PMID: 20877309
  9. results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain; an alternative model is proposed for the role of Tel1 in telomere homeostasis PMID: 20837994
  10. Deletion of SGS1 and the nuclease EXO1 reduces resection at uncapped telomeres and together with deletion of RAD9 permits cell survival without CDC13. PMID: 20808892
  11. Data present a structural, biochemical, and functional characterization of Cdc13. PMID: 20837709
  12. Mec1, the ATR ortholog, couples the detection of DNA ends with the inhibition of telomerase. Mec1 inhibits telomere healing by phosphorylating Cdc13 on its S306 residue, a phosphorylation event that suppresses Cdc13 accumulation at double-strand breaks PMID: 20194442
  13. the telomeric overhang is recognized with high affinity (3 pM) and sequence specificity by the essential protein Cdc13, which coordinates end-capping and telomerase activities at the telomere PMID: 16411763
  14. Neither Cdc13 nor its partner Stn1 are necessary for telomere capping if nuclease activities that are active at uncapped telomeres are attenuated. PMID: 16767084
  15. Results demonstrate that the telomerase recruitment domain of Cdc13p as an important new telomere-specific target of Mec1p/Tel1p. PMID: 17108359
  16. Lys568 is also located on the DNA-binding surface of Cdc13p PMID: 17166094
  17. Results suggest that the Cdc13 telomere cap coordinates Mec1 and Tel1 accumulation rather than simply covering the DNA ends at telomeres. PMID: 17377065
  18. identified distinct Stn1p domains that mediate interaction with either Ten1p or Cdc13p, allowing analysis of whether the interaction between Cdc13p and Stn1p is indeed essential for telomere capping or length regulation PMID: 17947422
  19. The binding of ssDNA to Cdc-13 induces localized conformational changes in a previously unappreciated region of the protein distant from the binding site. PMID: 18250086
  20. A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae PMID: 18845848
  21. Rad24-2 truncation protein affects telomere structure or recombination in a manner distinct from rad24-delta. PMID: 18948753
  22. Cdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 rather than the competing Stn1-Ten1 complex. PMID: 19135888

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Subcellular Location
Chromosome, telomere.
Database Links

KEGG: sce:YDL220C

STRING: 4932.YDL220C

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