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Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase, mitochondrial (RPO41), partial

Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase, mitochondrial (RPO41), partial

Code	CSB-YP319545SVG
Size	Pls inquire
Source	Yeast
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Code	CSB-EP319545SVG
Size	Pls inquire
Source	E.coli
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Code	CSB-EP319545SVG-B
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP319545SVG
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP319545SVG
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RPO41

Uniprot No.

P13433

Alternative Names

RPO41; YFL036W; DNA-directed RNA polymerase; mitochondrial; EC 2.7.7.6

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Gene References into Functions

Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA. PMID: 27311715
results thus indicate that the PPR motifs do not play an essential role in the function of the NTE domain of the mitochondrial RNA polymerase Rpo41p. PMID: 27908235
Herein the authors found that Rpo41 transcribes RNAs that can be extended by Mip1 on single and double-stranded DNA. PMID: 26184436
The study examined a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis. PMID: 26332125
This study identifies the importance of the +1+2 AA promoter sequence interactions with Rpo41-Mtf1 in dictating pre-transcribing complex formation, which regulates the binding of initiating NTPs and the catalytic efficiency of transcription initiation. PMID: 25249624
While Rpo41 induces a slight bend upon binding both the DNAs, addition of the Mtf1 results in severe bending of the promoter and unbending of the non-promoter DNA PMID: 21911502
Data show that he promoter DNA is sandwiched between Mtf1 and Rpo41 in the structural model. PMID: 20008320
Data show that both Rpo41p and Mtf1p contact with promoter DNA. PMID: 19920143
core mitochondrial RNA polymerase (Rpo41) has the intrinsic ability to initiate from promoters without its specificity factor (Mtf1) PMID: 15342628