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Recombinant Saccharomyces cerevisiae DNA ligase 1 (CDC9), partial

Recombinant Saccharomyces cerevisiae DNA ligase 1 (CDC9), partial

Code	CSB-YP012930SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP012930SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP012930SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP012930SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP012930SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CDC9

Uniprot No.

P04819

Alternative Names

CDC9; YDL164CDNA ligase 1; EC 6.5.1.1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. The mitochondrial form is required for mitochondrial DNA maintenance but is non-essential while the nuclear form is essential for cell viability.

Gene References into Functions

Rad27 lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. PMID: 19596905
Higher than normal levels of Cdc9p expression can yield genetic instability by disrupting the normal interplay of PCNA with other proteins such as Fen1. PMID: 15965249
Combining rad27-p with cdc9-p increases the incidence of repeat tract expansion frequency above the sum of the numbers recorded in the individual mutants PMID: 16079237
Recruitment of Saccharomyces cerevisiae Dnl4-Lif1 complex to a double-strand break requires interactions with Yku80 and the Xrs2 FHA domain. PMID: 18832348

Subcellular Location

[Isoform Mitochondrial]: Mitochondrion.; [Isoform Nuclear]: Nucleus.

Protein Families

ATP-dependent DNA ligase family

Database Links

KEGG: sce:YDL164C

STRING: 4932.YDL164C

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