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Recombinant Saccharomyces cerevisiae DNA repair protein RAD1 (RAD1), partial

Recombinant Saccharomyces cerevisiae DNA repair protein RAD1 (RAD1), partial

Code	CSB-YP356933SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP356933SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP356933SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP356933SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP356933SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RAD1

Uniprot No.

P06777

Alternative Names

RAD1; YPL022W; DNA repair protein RAD1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD10 forms an endonuclease that specifically degrades single-stranded DNA.

Gene References into Functions

One example occurred when yeast lacking RAD1 were exposed to cisplatin, and we characterized this interaction using whole-genome sequencing. Our sequencing results indicate mutagenesis by cisplatin in rad1Delta cells appeared to depend almost entirely on interstrand cross-links at GpCpN motifs. PMID: 28223526
low frequency of translocations observed in rad1-null single mutants PMID: 20012294
interchromosomal crossover recombination with short substrates is severely inhibited in the absence of RAD1 and is relatively insensitive to the presence of mismatches PMID: 16582436
mating-type switching in Saccharomyces cerevisiae strains defective in the Rad1-Rad10-Slx4 complex PMID: 18579504

Subcellular Location

Nucleus.

Protein Families

XPF family

Database Links

KEGG: sce:YPL022W

STRING: 4932.YPL022W

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