Recombinant Saccharomyces cerevisiae DNA repair protein RAD50 (RAD50), partial

Code CSB-YP019262SVG
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Source Yeast
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Code CSB-EP019262SVG
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Source E.coli
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Code CSB-EP019262SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019262SVG
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Source Baculovirus
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Code CSB-MP019262SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAD50
Uniprot No.
Alternative Names
RAD50; YNL250W; N0872; DNA repair protein RAD50; EC 3.6.-.-; 153 kDa protein
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific exonuclease activity. Rad50 provides ATP-dependent control of mre11 by unwinding and/or repositioning DNA ends into the mre11 active site.
Gene References into Functions
  1. Data demonstrate that the dimerization state of the Rad50 hook domain exerts diverse influences on Mre11 complex function. PMID: 25601756
  2. cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions PMID: 22885006
  3. deletion of RAD50 may block the major pathway of nonhomologous integration into a non-repetitive chromosomal locus; Rad50 may be involved in facilitating nonhomologous integration of both ends of the integrating DNA into a single chromosomal locus PMID: 21512733
  4. amino acid substitutions in the rad50 ATP binding domain have effects on DNA double strand break repair PMID: 15546877
  5. The Mre11/Rad50/Xrs2 (MRX) complex is essential for joining of incompatible ends by non-homologous end-joining during DNA repair, and the adenosine triphosphate (ATP)-dependent activities of Rad50 are critical for this process. PMID: 16043424
  6. Rad50 is required for the induction of recombination between homologous chromosomes in cells in the vegetative growth state upon exposure to methyl methanesulfonate. PMID: 17507781
  7. The Rad50/Mre11/Xrs2 complex is responsible for rapid processing of most damaged ends into substrates that subsequently undergo recombinational repair. PMID: 19763170

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Protein Families
SMC family, RAD50 subfamily
Database Links

KEGG: sce:YNL250W

STRING: 4932.YNL250W

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