Product Details

Purity

>85% (SDS-PAGE)

Target Names

RSP5

Uniprot No.

P39940

Alternative Names

RSP5; MDP1; NPI1; YER125W; SYGP-ORF41E3 ubiquitin-protein ligase RSP5; EC 2.3.2.26; HECT-type E3 ubiquitin transferase RSP5; Reverses SPT-phenotype protein 5

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.

Gene References into Functions

These data raised the possibility that Rsp5 enhances the function of Ena1/2/5 specifically in response to sodium acetate through an unknown mechanism other than ubiquitination of Rim8 and activation of Hog1-mediated signaling. PMID: 29106511
Authors present the molecular features in the methionine permease Mup1 that are required for Art1-Rsp5-mediated ubiquitination and endocytosis. PMID: 27798240
Rsp5 may be phosphorylated by PKA at position T761 and that this regulation is important for its localization and function. PMID: 26548973
subjecting yeast to proteotoxic stress, particularly thermal stress, triggers arresting related trafficking adaptor-Rsp5-mediated clearance of misfolded plasma membrane proteins. PMID: 23599894
Our findings suggest that stress-induced quality control is a common process requiring Rsp5p for plasma membrane proteins in yeast. PMID: 25001409
Rsp5 and its mammalian homologue Nedd4 are important E3 ligases responsible for the increased ubiquitylation induced by heat stress. PMID: 25344756
SUMOylation regulates the homologous to E6-AP carboxyl terminus (HECT) ubiquitin ligase Rsp5p. PMID: 23443663
In one highly sensitive cluster on the surface of ubiquitin, almost every amino acid substitution caused growth defects. PMID: 23376099
our results show that Rsp5 ligase plays a role in regulating retrograde Golgi-to-ER trafficking. PMID: 22761830
Rsp5 together with Lsb1, Lsb2 and Sla1 regulate the level of Las17, an important activator of actin polymerization. PMID: 22000681
Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals PMID: 21868125
suggest that functional Rsp5p is required to maintain the integrity of cytoplasmic ribosomes under rich nutrient conditions PMID: 21665996
Results support a model in which the N-lobe-binding site acts to localize and orient the distal end of the ubiquitin chain to promote conjugation of the next ubiquitin molecule. PMID: 21399621
Bsd2 is a three-transmembrane domains protein with a PPXY motif that binds the HECT domain ubiquitin ligase Rsp5 PMID: 14988731
Rsp5p plays a novel and key role in intracellular trafficking, and extend the currently very short list of substrates ubiquitylated in vivo by several different ubiquitin ligases acting cooperatively PMID: 15086787
Rsp5 is important for recovery from heat-induced protein synthesis arrest PMID: 15527788
Counteraction of PUT4 downregulation by npi1 mutation that affects cellular ubiquitination function is reported. PMID: 15973048
results suggest that enhanced phosphatidylinositol synthesis may account for PIS1 suppression of rsp5 defects PMID: 16363994
Sequential assembly of first a Bsd2/Rsp5 complex, then a Tre1/Bsd2/Rsp5 complex followed by a rearrangement of PY-WW interactions is required for the ubiquitination of Smf1. PMID: 17429078
identification of numerous known and novel ubiquitinated substrates of this E3 ligase PMID: 17551511
Rsp5 ubiquitin ligase is required for vacuolar targeting and degradation of high-affinity copper transporter Ctr1p fused to green fluorescent protein, following the endocytic downregulation pathway requiring delivery to the vacuole. PMID: 17645432
A combination of yeast proteome chip assays, genetic screening, and in vitro/in vivo biochemical analyses was used to identify and characterize eight novel in vivo substrates of the ubiquitinating enzyme Rsp5. PMID: 17951556
turnover of the low affinity P i transporters is initiated by Rsp5-mediated ubiquitination followed by internalization and degradation by the endocytic pathway. PMID: 18165238
lysine residues 983 and 985 contained within the carboxy-terminal domain of Mga2p120 are Rsp5p-directed Ub-conjugation sites PMID: 19061897
Rsp5p plays an important role in the post-transcriptional fate of mRNA under nutrient starvation. PMID: 19180642
Point mutations that disrupt ubiquitin binding also result in temperature-sensitive growth defects in yeast, indicating that the Rsp5 ubiquitin-binding site is important for Rsp5 function in vivo PMID: 19252184
inhibits tombavirus replication via p92 replication protein and replicase PMID: 19759160

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Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, actin patch.

Protein Families

RSP5/NEDD4 family

Database Links

KEGG: sce:YER125W

STRING: 4932.YER125W

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Code	CSB-YP334646SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP334646SVG
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Code	CSB-EP334646SVG-B
MSDS	MSDS
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Code	CSB-BP334646SVG
MSDS	MSDS
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Code	CSB-MP334646SVG
MSDS	MSDS
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Source	Mammalian cell
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Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase RSP5 (RSP5), partial

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