Recombinant Saccharomyces cerevisiae Helicase SEN1 (SEN1), partial

Code CSB-YP311463SVG
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Source Yeast
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Code CSB-EP311463SVG
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Source E.coli
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Code CSB-EP311463SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311463SVG
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Source Baculovirus
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Code CSB-MP311463SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SEN1
Uniprot No.
Alternative Names
SEN1; YLR430W; L9576.1; Helicase SEN1; EC 3.6.4.-; tRNA-splicing endonuclease positive effector
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair.
Gene References into Functions
  1. The helicase domain (HD) is sufficient to dissociate the elongation complex (EC) in vitro. Both full-length Sen1 and its HD can translocate along single-stranded RNA and DNA in the 5 to 3 direction. PMID: 28180347
  2. we identify new targets and functions of Sen1-dependent termination, including a role in repression of meiotic genes in vegetative cells. In combination with other recent whole-genome studies on termination of non-coding RNAs, our results provide promising directions for further investigation. PMID: 28665995
  3. The authors also show that subdomain 1C (the "prong") is an essential element for 5'-3' unwinding of RNA and for Sen1-mediated transcription termination in vitro. PMID: 28408439
  4. DNA/RNA helicase Sen1 mutant causes modulation of protein-serine-threonine kinase TOR pathway and autophagy. PMID: 27718307
  5. Data indicate that mutant of the RNA processing machinery (RNT1), and RNA/DNA helicase SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype. PMID: 26364722
  6. results are consistent with a model in which Sen1 promotes transcription termination by resolving R-loops. PMID: 26198638
  7. We provide evidence that this topological stress contributes to recruit and/or stabilize condensin at Pol III-transcribed genes in the absence of Swd2.2 and Sen1 PMID: 25392932
  8. Multiple domains of Sen1 are involved in transcription termination. PMID: 25116135
  9. Sen1p recognizes the nascent RNA & hydrolyzes ATP to dissociate the elongation complex. Sen1p-dependent transcription termination is highly specific & does not require the C-terminal domain of RNAPII. PMID: 23748379
  10. Study shows that the Sen1/Senataxin DNA/RNA helicase associates with forks, promoting their progression across RNA polymerase II (RNAPII)-transcribed genes. PMID: 23141540
  11. Analysis of a homogeneous set of several hundreds of artificial, Nrd1-Nab3-Sen1 complex-dependent terminators revealed novel and extended sequence determinants for transcription termination, as well as supermotifs critical for termination. PMID: 23032188
  12. transcriptome-wide distribution of termination factors Nrd1, Nab3, and Sen1 is reported. PMID: 22028667
  13. show here that Nrd1, Nab3, and Sen1 bind to a number of noncoding RNAs in an unexpected manner PMID: 21954178
  14. Data suggest that Set1 promotes proper early termination by the Nrd1-Nab3-Sen1 complex by affecting the kinetics of Pol II transcription in early elongation. PMID: 21709022
  15. Findings reveal a regulatory mechanism in which Mpk1 overcomes transcriptional attenuation by blocking recruitment of the Sen1-Nrd1-Nab3 termination complex to the elongating polymerase. PMID: 21376235
  16. Sen1p-Rpb1p and Sen1p-Rnt1p interactions occur independently of each other and serve genetically separable purposes in targeting Sen1p to function in two temporally overlapping steps in gene expression. PMID: 19884310
  17. the Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain PMID: 18660819

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Subcellular Location
Nucleus.
Protein Families
DNA2/NAM7 helicase family
Database Links

KEGG: sce:YLR430W

STRING: 4932.YLR430W

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