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Recombinant Saccharomyces cerevisiae Low glucose sensor SNF3 (SNF3), partial

Recombinant Saccharomyces cerevisiae Low glucose sensor SNF3 (SNF3), partial

Code	CSB-YP319940SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP319940SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP319940SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP319940SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP319940SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SNF3

Uniprot No.

P10870

Alternative Names

SNF3; YDL194W; D1234; Low glucose sensor SNF3; High-affinity glucose receptor SNF3; High-affinity transporter-like sensor SNF3; Sucrose nonfermenting protein 3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

High-affinity low glucose sensor that is part of the sensor/receptor-repressor (SSR) glucose-signaling pathway, which detects extracellular glucose and induces expression of glucose transporters that bring glucose into the cell. The transporter-like sensor generates an intracellular signal in the presence of low levels of glucose to promote low glucose-induced expression of HXT2. Binding of glucose to the SNF3 transmembrane domain activates a downstream signaling cascade, leading to phosphorylation of the RGT1 corepressors MTH1 and STD1, targeting them for SCF(Grr1)-dependent ubiquitination and degradation. Depletion of the corepressors robs RGT1 of its ability to repress expression of HXT genes, leading to accumulation of glucose transporters in the plasma membrane. SNF3 is involved as well in the transport of mannose and fructose. Even though SNF3 is similar to glucose transporters, it appears to be unable to transport glucose.

Gene References into Functions

Rgt2 and Snf3 glucose sensors are primary targets of methylglyoxal, which causes degradation of the Rgt2 and Snf3. PMID: 26764094
Mth1 and Std1, which are downstream effectors of the Snf3/Rgt2 pathway, were required for viability through mitochondrial function but not fermentative metabolism PMID: 25541485
Snf3 apparent affinity for glucose was lowest when the intracellular glucose concentration was high. The results conform to an alternating-access model for transporter-like sensors PMID: 20564191
This study reveals that Snf3, in addition to glucose, also senses fructose and mannose, as well as the glucose analogues 2-deoxyglucose, 3-O-methylglucoside and 6-deoxyglucose. PMID: 20014043
Suppression of the snf3 mutant phenotype by deletion of IRA2 was confirmed. PMID: 17311585

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family

Database Links

KEGG: sce:YDL194W

STRING: 4932.YDL194W

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