Product Details

Purity

>85% (SDS-PAGE)

Target Names

KAP95

Uniprot No.

Q06142

Alternative Names

KAP95; YLR347C; L8300.15Importin subunit beta-1; Importin-95; Karyopherin subunit beta-1; Karyopherin-95

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo. Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of histones H2A and H2B. Mediates the nuclear import of transcription factor GCN4.

Gene References into Functions

Data suggest that, in nuclear exosomes, karyopherins are bound to the Rrp6 complex; Srp1, Kap95, and Sxm1 appear to be the most important karyopherins for nuclear import of Rrp6. (Rrp6 = exosome nuclease subunit RRP6; Srp1 = karyopherin alpha kap60; Kap95 = karyopherin beta Kap95; Sxm1 = karyopherin Kap108) PMID: 28539363
The Kap95 is able to mediate efficient Mig2-Kap95 interaction in the absence of the importin-alpha. PMID: 23131016
Hxk2 as a new cargo for the alpha/beta-importin pathway of S. cerevisiae. PMID: 22157003
Kap95 is likely the primary karyopherin responsible for Replication protein A nuclear import in yeast. PMID: 21332387
The authors identified karyopherin Kap95 as the transport factor responsible for the nuclear import of Slt2 and Rlm1. PMID: 19930464
identification of a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p PMID: 15864302
There is a correlation between the binding affinity of an NLS cargo for the NLS receptor, importin alpha, and the import rate for this cargo. PMID: 16785238
Kap95 serves as a key regulator of nuclear pore complex assembly into intact nuclear envelopes. PMID: 17182855
Data show the crystal structure of the Kap95p-RanGDP complex shows that Kap95p induces the switch I and II regions of RanGDP to adopt a conformation that resembles that of the GTP-bound form. PMID: 18708071
Pct1 interacts with Kap60 and Kap95 with this tripartite complex transiting the nuclear pore. PMID: 19141610

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, nuclear pore complex.

Protein Families

Importin beta family, Importin beta-1 subfamily

Database Links

KEGG: sce:YLR347C

STRING: 4932.YLR347C

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Code	CSB-YP012490SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP012490SVG
MSDS	MSDS
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Code	CSB-EP012490SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP012490SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP012490SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Recombinant Saccharomyces cerevisiae Importin subunit beta-1 (KAP95), partial

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