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Recombinant Saccharomyces cerevisiae Iron-regulated transcriptional activator AFT1 (AFT1), partial

Recombinant Saccharomyces cerevisiae Iron-regulated transcriptional activator AFT1 (AFT1), partial

Code	CSB-YP324340SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP324340SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP324340SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP324340SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP324340SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

AFT1

Uniprot No.

P22149

Alternative Names

AFT1; RCS1; YGL071WIron-regulated transcriptional activator AFT1; Activator of iron transcription protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Probable transcription factor that activates the genes for FRE1, FRE2 and FET3 in response to iron deprivation. Also required for the expression of LSO1. Iron could interact directly with AFT1 and inhibits its activity.

Gene References into Functions

Taken together, these results suggest that the protein-protein interaction between Sit1 and Aft1 is an important factor in the ferrioxamine B uptake activity of Sit1. PMID: 26323600
Aft1p dissociates from its target promoters under iron-replete conditions due to an interaction between Aft1p and the monothiol glutaredoxin Grx3p or Grx4p (Grx3/4p). PMID: 23045394
These data suggest that Aft1p and Mac1p might corporately regulate transcription of CTR2. PMID: 22683637
investigation of role of Aft-1 (and YAH1) in regulation of iron metabolism under various growth conditions (e.g., low iron in media) PMID: 21361388
Aft1/Aft2 activation of the iron regulon depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis PMID: 15649888
different cis-regulatory elements are found for Aft1 and Aft2, and transcription analysis indicates that Aft1 is more specific for the canonical iron-responsive element TGCACCC than is Aft2. PMID: 16024809
These findings suggest that cisplatin activates Aft1 through the inhibition of iron uptake into the cells, after which the expression of Aft1 target genes involved in iron uptake might be induced. PMID: 17096368
These results suggest that iron induces a conformational change in Aft1p, in which Aft1p Cys291 plays a critical role, and that, in turn, Aft1p is recognized by Msn5p and exported into the cytoplasm in an iron-dependent manner. PMID: 17538022
These results suggest that Aft1 interacts with Arn3 and may regulate the ubiquitination of Arn3 in the cytosolic compartment. PMID: 19469713