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Recombinant Saccharomyces cerevisiae Phosphatidylinositol 3-kinase VPS34 (VPS34), partial

Recombinant Saccharomyces cerevisiae Phosphatidylinositol 3-kinase VPS34 (VPS34), partial

Code	CSB-YP334903SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP334903SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP334903SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP334903SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP334903SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

VPS34

Uniprot No.

P22543

Alternative Names

VPS34; END12; PEP15; VPL7; VPT29; YLR240W; L9672.10; Phosphatidylinositol 3-kinase VPS34; PI3-kinase VPS34; PI3K VPS34; PtdIns-3-kinase VPS34; EC 2.7.1.137; Carboxypeptidase Y-deficient protein 15; Vacuolar protein sorting-associated protein 34; Vacuolar protein-targeting protein 29

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity.

Gene References into Functions

Data indicate that class Ia Phosphatidylinositol 3-Kinase p85alpha germ line mutations associated with SHORT syndrome did not increase PI3K activity in the yeast model. PMID: 28143957
mutants lacking Vps34 display multiple phenotypes indicating impaired transcription elongation. PMID: 23335340
VPS34 plays a critical role in the decline of extracellular FBPase in response to glucose. PMID: 22833678
Kes1-mediated regulation of the autophagy/cytoplasm to the vacuole trafficking pathway is prevented by increasing expression of the PI3K Vps34 PMID: 20729555
Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate. PMID: 16839886
Study shows that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P. PMID: 18533003