Recombinant Saccharomyces cerevisiae Protein STN1 (STN1)

Code CSB-YP330958SVG
MSDS
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Source Yeast
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Code CSB-EP330958SVG
MSDS
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Source E.coli
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Code CSB-EP330958SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330958SVG
MSDS
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Source Baculovirus
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Code CSB-MP330958SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STN1
Uniprot No.
Alternative Names
STN1; YDR082W; D4456; YD8554.15; Protein STN1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-494
Target Protein Sequence
MDKYGHIAHQ EGDVCYYIPR LFKYNSYYSG TEDVRIFVGD LKYRMRVSLQ ICEKYYDRRL SMLFWKNHPL QQIHLIGCII GLQFKWIGKQ EYIFFQLDDC TSDSSLVGYT SDMRFLTCKV KKDSILSWGL NITDLIGLTL HVYGQASLNY QELQVEYLRL CYSLTEEIDH WKITMNMREQ LDTPWSLSDF VIGELFTQEQ EWTPETSQIE VVNPDFVGIG YKTPESKRNE TTFIEQLQEE RLKDELEIIS PYNSTDTSNS VHSLSFRFVS SLKDFPETHF LNSGDQIDNG NDEQLKKLEY QSANLPVMIP NRTSAKSNLM LILLGLQMKE ISNSDLYKLK EVRSVVTSLA SFLFQQQNVG VMKSFDSLEK EAFRDLVNRL VSQGLIGLKD KTSETFDLLP LKNLFEYAEK RISVLMKLQC YTGTVQLSHV QEKLHLPYIT TNGIVDVFKE CLKRTKKQYP EVLKNWWIDL DPKNGMEDQN SGILLHLEYA AAYS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13.
Gene References into Functions
  1. Stn1 overrides DNA replication control through the S phase checkpoint PMID: 19171895
  2. The N terminus of Stn1 interacts with Ten1 and carries out its essential capping function. The C terminus of Stn1 binds both Cdc13 and Pol12. PMID: 19172739
  3. the crystal structures of the C-terminal domain of the Saccharomyces cerevisiae Stn1 and the Schizosaccharomyces pombe Ten1 proteins are reported. PMID: 19884503
Subcellular Location
Chromosome, telomere.
Database Links

KEGG: sce:YDR082W

STRING: 4932.YDR082W

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