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Recombinant Saccharomyces cerevisiae Spindle pole body-associated protein VIK1 (VIK1)

Recombinant Saccharomyces cerevisiae Spindle pole body-associated protein VIK1 (VIK1)

Code	CSB-YP606287SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP606287SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP606287SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP606287SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP606287SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

VIK1

Uniprot No.

Q12045

Alternative Names

VIK1; YPL253C; Spindle pole body-associated protein VIK1; Vegetative interaction with KAR3 protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Targets and/or maintains KAR3 at the spindle pole body during vegetative growth.

Gene References into Functions

Neck rotation and neck mimic docking in Vik1 and Cik1 is a structural mechanism for communication with Kar3. PMID: 23043140
Rapid ATP binding to Kar3 is associated with rotation of the coiled-coil stalk, and the postpowerstroke ATP hydrolysis is independent of Vik1. PMID: 22977241
show through structural determination of a C-terminal heterodimeric Kar3Vik1, electron microscopy, equilibrium binding, and motility that at the start of the cycle, Kar3Vik1 binds to or occludes two alphabeta-tubulin subunits on adjacent protofilaments PMID: 22734002
Vik1 binds more tightly to microtubules than Kar3 and facilitates cooperative microtubule decoration by Kar3/Vik1 heterodimers, and yet allows motility. PMID: 17382884

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Nucleus.

Database Links

KEGG: sce:YPL253C

STRING: 4932.YPL253C

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