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Recombinant Saccharomyces cerevisiae Transcription factor RLM1 (RLM1), partial

Recombinant Saccharomyces cerevisiae Transcription factor RLM1 (RLM1), partial

Code	CSB-YP623592SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP623592SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP623592SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP623592SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP623592SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RLM1

Uniprot No.

Q12224

Alternative Names

RLM1; YPL089C; LPG19C; Transcription factor RLM1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

May function as a transcription factor downstream of MPK1 that is subject to activation by the MPK1 mitogen-activated protein kinase pathway. Binds to the DNA sequence 5'-CTA[TA](4)TAG-3'. At least some RML1 target genes are involved in cell wall biosynthesis.

Gene References into Functions

Rlm1 regulates the transition from G1 to S. rlm1Delta mutant forms cell groups in which a mother cell is surrounded by smaller "satellite-daughter" cells. rlm1Delta satellite daughters were unable to grow or divide further. PMID: 28637712
this paper shows that, under cell wall stress conditions, Rlm1 interacts with Rlm1-binding domains at the promoter regions of RLM1 and SLT2, eliciting transcriptional positive-feedback mechanisms PMID: 26933180
RLM1 deletion decreases the transcription of SLT2 encoding the Mpk1p kinase. PMID: 21326366
during cell wall stress, the interaction of Slt2p with Cdc37p and Hsp90 is crucial for Slt2p-dependent downstream responses, such as the activation of the transcription factor Rlm1p PMID: 17220467

Subcellular Location

Nucleus.

Protein Families

MEF2 family

Database Links

KEGG: sce:YPL089C

STRING: 4932.YPL089C

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