Recombinant Saccharomyces cerevisiae Transcriptional activator protein CUP2 (CUP2)

Code CSB-YP322767SVG
MSDS
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Source Yeast
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Code CSB-EP322767SVG
MSDS
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Source E.coli
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Code CSB-EP322767SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322767SVG
MSDS
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Source Baculovirus
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Code CSB-MP322767SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CUP2
Uniprot No.
Alternative Names
CUP2; ACE1; YGL166W; G1810Transcriptional activator protein CUP2; Copper-fist transcription factor
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-225
Target Protein Sequence
MVVINGVKYA CETCIRGHRA AQCTHTDGPL QMIRRKGRPS TTCGHCKELR RTKNFNPSGG CMCASARRPA VGSKEDETRC RCDEGEPCKC HTKRKSSRKS KGGSCHRRAN DEAAHVNGLG IADLDVLLGL NGRSSDVDMT TTLPSLKPPL QNGEIKADSI DNLDLASLDP LEQSPSISME PVSINETGSA YTTTNTALND IDIPFSINEL NELYKQVSSH NSHSQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Trans-acting regulatory protein that activates transcription of the CUP1 gene (metallothionein) in response to copper ions. Binds to the CUP1 UAS sequence 5'-GCTTCTTTTCCGCTGA-3'. Binds DNA only in presence of copper or silver. Copper seems to alter the conformation of the protein.
Gene References into Functions
  1. A characteristic residence time for non-specific interactions of Ace1 transcription factor with chromatin PMID: 27566148
  2. The induction of CUP1 requires the presence of Ace1p and the binding of Ace1p at the CUP1 promoter during activation provides evidence that Ace1p is directly involved in CUP1 induction. PMID: 22609398
  3. Results show that metalloregulation of Ace1 and Mac1 occurs independently. PMID: 16278453
  4. findings report that fast & slow cycling of ACE1 can occur simultaneously on an endogenous promoter & that slow cycling in this system reflects an oscillation in the fraction of accessible promoters rather than recruitment & release of stably bound ACE1 PMID: 18218898
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YGL166W

STRING: 4932.YGL166W

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