Recombinant Saccharomyces cerevisiae AP-1-like transcription factor YAP5 (YAP5)

Code CSB-YP340276SVG
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Source Yeast
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Code CSB-EP340276SVG
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Source E.coli
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Code CSB-EP340276SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340276SVG
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Source Baculovirus
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Code CSB-MP340276SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YAP5
Uniprot No.
Alternative Names
YAP5; YIR018W; AP-1-like transcription factor YAP5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-245
Target Protein Sequence
MALPLIKPKE SEESHLALLS KIHVSKNWKL PPRLPHRAAQ RRKRVHRLHE DYETEENDEE LQKKKRQNRD AQRAYRERKN NKLQVLEETI ESLSKVVKNY ETKLNRLQNE LQAKESENHA LKQKLETLTL KQASVPAQDP ILQNLIENFK PMKAIPIKYN TAIKRHQHST ELPSSVKCGF CNDNTTCVCK ELETDHRKSD DGVATEQKDM SMPHAECNNK DNPNGLCSNC TNIDKSCIDI RSIIH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator involved in the regulation of genes expressed in response to environmental changes and metabolic requirements. According to genome-wide promoter binding and gene expression studies it is a coregulator for the expression of ribosomal genes, while its own expression is induced by the cell cycle specific activator SBF (SWI4-SWI6).
Gene References into Functions
  1. Fe/S cluster binding to Yap5-activator domain induces a conformational change that may activate transcription PMID: 25368382
  2. loss of activity of the cytosolic iron-sulfur cluster assembly complex proteins or deletion of cytosolic glutaredoxins did not reduce expression of Yap5 target genes PMID: 22915593
  3. we propose that under Fe-adequacy Yap5 is activated leading to the upregulation of CCC1 with the consequent accumulation of Fe in the vacuole and deprivation of the cytosolic iron pool. PMID: 22616008
  4. the Yap5-mediated induction of TYW1 provides protection from high iron toxicity by the consumption of free cytosolic iron through the formation of protein-bound iron-sulfur clusters PMID: 21917924
  5. These results show that Yap5 is an iron-sensing transcription factor and that iron regulates transcriptional activation. PMID: 18070921

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
BZIP family, YAP subfamily
Database Links

KEGG: sce:YIR018W

STRING: 4932.YIR018W

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