Recombinant Saccharomyces cerevisiae Actin-binding protein(ABP1) ,partial

Code CSB-YP322813SVG
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Source Yeast
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Code CSB-EP322813SVG
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Source E.coli
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Code CSB-EP322813SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322813SVG
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Source Baculovirus
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Code CSB-MP322813SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ABP1
Uniprot No. P15891
Alternative Names ABP1; YCR088W; YCR88WActin-binding protein
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Regulates ARP2/3 complex-mediated actin assembly. Recruits ARP2/3 complex to sides of preexisting actin filaments, which may promote nucleation or stabilization of filament branches. Binds to actin filaments, but not actin monomers. Actin binding is required for ARP2/3 complex activation. May also have a role in linking the actin cytoskeleton to endocytosis. recruits components of the endocytotic machinery to cortical actin patches, known sites of endocytosis.
Gene References into Functions
  1. a structural and functional view of the Abp1-actin interaction and demonstration that Abp1 has distinct cellular roles as an adapter, linking different sets of ligands for each function PMID: 15872087
  2. These data provide the first evidence of biological relevance for any Abp1p SH3 domain-mediated interaction. PMID: 17409071
  3. Data imply that the AbpSH3 must bind extended target sites to function efficiently inside the cell. PMID: 19590096
Subcellular Location Cytoplasm, cytoskeleton, actin patch
Protein Families ABP1 family
Database Links

KEGG: sce:YCR088W

STRING: 4932.YCR088W

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