Recombinant Saccharomyces cerevisiae Beta-glucan synthesis-associated protein KRE6 (KRE6), partial

Code CSB-YP341327SVG
MSDS
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Source Yeast
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Code CSB-EP341327SVG
MSDS
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Source E.coli
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Code CSB-EP341327SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341327SVG
MSDS
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Source Baculovirus
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Code CSB-MP341327SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KRE6
Uniprot No.
Alternative Names
KRE6; CWH48; YPR159WBeta-glucan synthesis-associated protein KRE6; Killer toxin-resistance protein 6
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the synthesis of (1->6)- and (1->3)-beta-D-glucan polymers of the yeast cell wall in vivo. It is required for full activity of beta-glucan synthase in vitro. May be involved in the maturation and transport of cell wall proteins (CWP) to the cell wall. May act as a transglucosidase and contribute to the construction of a protein-bound glucan-structure that acts as an acceptor site for the addition of (1->6)-beta-D-glucan at the cell surface.
Gene References into Functions
  1. the action of multiple ER chaperon-like proteins is required for proper folding and localization of Kre6 and probably Skn1 to function in beta-1,6-glucan synthesis. PMID: 22447934
  2. This is the first evidence of a beta-1,6-glucan-related protein, Kre6, showing the polarized membrane localization that correlates with its biological function PMID: 21193403
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
SKN1/KRE6 family
Database Links

KEGG: sce:YPR159W

STRING: 4932.YPR159W

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