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Recombinant Saccharomyces cerevisiae Cell cycle protein kinase DBF2 (DBF2), partial

Recombinant Saccharomyces cerevisiae Cell cycle protein kinase DBF2 (DBF2), partial

Code	CSB-YP322112SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP322112SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP322112SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP322112SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP322112SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DBF2

Uniprot No.

P22204

Alternative Names

DBF2; YGR092WCell cycle protein kinase DBF2; EC 2.7.11.1; Dumbbell forming protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Ser/Thr-protein kinase involved in the mitotic exit network (MEN) and required after the metaphase to anaphase cell cycle transition. Phosphorylates CHS2 to regulate its dynamics and chitin synthesis at the division site during cytokinesis. Coordinates septin and actomyosin ring (AMR) functions during cytokinesis through the phosphorylation of HOF1. In complex with MOB1, phosphorylates CDC14 at sites adjacent to its nuclear localization sequence, thereby retaining CDC14 in the cytoplasm. Binds also to SWI5 and CLB2 mRNAs cotranscriptionally to regulate their decay. In the nucleus, the DBF2-MOB1 complex regulates passenger protein localization during anaphase. Mediates sorbic acid stress tolerance through promoting vacuolar H(+)-ATPase function, probably through phosphorylation of VMA1 and VMA2 subunits.

Gene References into Functions

y usage of an enhanced system for sporulation-induced protein depletion, we demonstrate that the core mitotic exit network (MEN) is involved in age-based SPB selection. Moreover, efficient genome inheritance requires Dbf2/20-Mob1 during a late step in spore maturation. PMID: 28450458
Dbf2 coordinates septin and actomyosin ring functions during cytokinesis through the regulation/control of Hof1. PMID: 23447700
Dbf2 regulates primary septum formation through at least two independent pathways PMID: 22573892
Data show that polo-like kinase Cdc5 first phosphorylates Hof1 to allow subsequent phosphorylation by Dbf2-Mob1. PMID: 21498574
required for establishing passenger protein localization on the mitotic spindle PMID: 16176976

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Bud neck. Nucleus. Note=Localizes on spindle ole body (SPB) for much of the cell cycle and migrates from there to the bud neck in late mitosis. SPB localization during anaphase coincides with activation kinase activity and depends on TEM1 and CDC15. In absence of BUB2, localizes to SPB in cell cycle stages other than anaphase and telophase.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: sce:YGR092W

STRING: 4932.YGR092W

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