Recombinant Saccharomyces cerevisiae Cop9 signalosome complex subunit 12 (CSN12)

Code CSB-YP341670SVG
MSDS
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Source Yeast
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Code CSB-EP341670SVG
MSDS
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Source E.coli
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Code CSB-EP341670SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341670SVG
MSDS
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Source Baculovirus
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Code CSB-MP341670SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSN12
Uniprot No.
Alternative Names
CSN12; YJR084W; J1860Cop9 signalosome complex subunit 12
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-423
Target Protein Sequence
MDVDIGCYFE EKRYDDKLLD FIRYDVKTPK KTKYILQRPT ATDEESVRLQ RFYQLGVDLK LKYSKRRSLK KQGRIKNATE ELLRLANEQL KLFNRIVERE TNWIIYPLWV MAKQLIRLAN ESSELNKDSI EECGRTIHRS FTICLNDRNP RLNENKKIGC YMFANLEFSI YHRLSNKDMI KNLVKVLESR VNARDIPPLN KSLAMEHKSQ VVLYNYYLGQ YYGCLENDHE RGFFHLNEAL LQCPMLYVES TGKFVLQGQM EKIMILLVPL ALLTKRLYPH WDHPVIAGVI TRSKRLSQVY PTLVRSVISG NLSLYEATAA SHERFFLSQG LHVVITLLRE VVFTRLVQRC WQWGNDRKSI MPLKILLATK QHDSSANEDE EEQLDALECR LASAIASGLL RAYLSHSNRC IVFSKKEPFP HSK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the COP9 signalosome (CSN) complex that acts as an regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunit of SCF-type E3 ubiquitin-protein ligase complexes. The CSN complex is involved in the regulation of the mating pheromone response. CSN12 forms a complex with THP3 that is recruited to transcribed genes and required for transcription elongation.
Gene References into Functions
  1. role in transcription elongation for Ypr045c (Thp3) and the Csn12 component of the COP9 signalosome PMID: 21149575
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
CSN12 family
Database Links

KEGG: sce:YJR084W

STRING: 4932.YJR084W

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