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Recombinant Saccharomyces cerevisiae DNA polymerase gamma (MIP1), partial

Recombinant Saccharomyces cerevisiae DNA polymerase gamma (MIP1), partial

Code	CSB-YP322240SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP322240SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP322240SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP322240SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP322240SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MIP1

Uniprot No.

P15801

Alternative Names

MIP1; YOR330CDNA polymerase gamma; EC 2.7.7.7; Mitochondrial DNA polymerase catalytic subunit

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the replication of mitochondrial DNA.

Gene References into Functions

Yeast Mip1, the mitochondrial DNA polymerase gamma, harbors a conserved C-terminal extension required for maintenance of mitochondrial DNA in vivo. PMID: 16491467
Our study also further confirms the utility of yeast Mip1p as a tool for studying human DNA polymerase (pol gamma)variants. PMID: 26077851
Disrupting exonuclease activity of mutant variants was not associated with increased spontaneous mutagenesis compared with exonuclease-proficient alleles, suggesting that most or all of the mtDNA was replicated by wild type Mip1. PMID: 25340760
disruption of Mip1 exonuclease activity increases mtDNA deletions 160-fold. PMID: 23589460
Point mutations in the 3'-5' exonuclease domain of Mip1 result in severe impairment of the polymerase function and DNA binding. PMID: 20601675
mip1 containing mutations associated with mitochondrial disease causes mutagenesis and depletion of mtDNA in Saccharomyces cerevisiae. PMID: 20185557
Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains. PMID: 17720904
The yeast mitochondrial DNA (mtDNA) replicase Mip1 has been used as a model to generate five mutations equivalent to POLG mutations associated with a broad spectrum of diseases in human. PMID: 17980715