Recombinant Saccharomyces cerevisiae DNA polymerase gamma(MIP1) ,partial

Code CSB-YP322240SVG
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Source Yeast
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Code CSB-EP322240SVG
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Source E.coli
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Code CSB-EP322240SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322240SVG
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Source Baculovirus
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Code CSB-MP322240SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MIP1
Uniprot No. P15801
Alternative Names MIP1; YOR330CDNA polymerase gamma; EC 2.7.7.7; Mitochondrial DNA polymerase catalytic subunit
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Involved in the replication of mitochondrial DNA.
Gene References into Functions
  1. Yeast Mip1, the mitochondrial DNA polymerase gamma, harbors a conserved C-terminal extension required for maintenance of mitochondrial DNA in vivo. PMID: 16491467
  2. Our study also further confirms the utility of yeast Mip1p as a tool for studying human DNA polymerase (pol gamma)variants. PMID: 26077851
  3. Disrupting exonuclease activity of mutant variants was not associated with increased spontaneous mutagenesis compared with exonuclease-proficient alleles, suggesting that most or all of the mtDNA was replicated by wild type Mip1. PMID: 25340760
  4. disruption of Mip1 exonuclease activity increases mtDNA deletions 160-fold. PMID: 23589460
  5. Point mutations in the 3'-5' exonuclease domain of Mip1 result in severe impairment of the polymerase function and DNA binding. PMID: 20601675
  6. mip1 containing mutations associated with mitochondrial disease causes mutagenesis and depletion of mtDNA in Saccharomyces cerevisiae. PMID: 20185557
  7. Mip1p contains a single nucleotide polymorphism changing the strictly conserved threonine 661 to alanine. This substitution is responsible for the increased rate of mitochondrial DNA point mutations and deletions in these strains. PMID: 17720904
  8. The yeast mitochondrial DNA (mtDNA) replicase Mip1 has been used as a model to generate five mutations equivalent to POLG mutations associated with a broad spectrum of diseases in human. PMID: 17980715

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Subcellular Location Mitochondrion
Protein Families DNA polymerase type-A family
Database Links

KEGG: sce:YOR330C

STRING: 4932.YOR330C

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