Recombinant Saccharomyces cerevisiae E3 SUMO-protein ligase MMS21 (MMS21)

Code CSB-YP336471SVG
MSDS
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Source Yeast
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Code CSB-EP336471SVG
MSDS
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Source E.coli
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Code CSB-EP336471SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336471SVG
MSDS
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Source Baculovirus
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Code CSB-MP336471SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMS21
Uniprot No.
Alternative Names
MMS21; NSE2; YEL019C; E3 SUMO-protein ligase MMS21; EC 2.3.2.-; E3 SUMO-protein transferase MMS21; Methyl methanesulfonate-sensitivity protein 21; Non-structural maintenance of chromosome element 2; Non-SMC element 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-267
Target Protein Sequence
MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI MELRCKIAKM KESQEQDKRS SQAIDVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
Gene References into Functions
  1. Mms21 prevents the accumulation of spontaneous DNA lesions that cause diverse gross chromosomal rearrangements. PMID: 29505562
  2. Data indicate that Snf1 Is the target of SUMO (E3) ligase Mms21. PMID: 24108357
  3. Deletion of E3 ligase domain of Mms21 led to decreased binding of Sir2p, Sir3p and Sir4 at subtelomeric chromatins and increased H3K4 tri-methylation at telomere-distal euchromatin regions. PMID: 23911609
  4. Small ubiquitin-related modifier ligase activity of Mms21 is required for maintenance of chromosome integrity during the unperturbed mitotic cell division cycle in Saccharomyces cerevisiae PMID: 21324902
  5. Mms21 requires the Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription) RING domain to function as a SUMO ligase. PMID: 15738391
  6. Molecular cloning proved pso10-1 to be a mutant allele of gene MMS21 that encodes a SUMO ligase involved in the sumoylation of several DNA repair proteins. PMID: 18437386

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
NSE2 family
Database Links

KEGG: sce:YEL019C

STRING: 4932.YEL019C

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