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Recombinant Saccharomyces cerevisiae EH domain-containing and endocytosis protein 1 (EDE1), partial

Recombinant Saccharomyces cerevisiae EH domain-containing and endocytosis protein 1 (EDE1), partial

Code	CSB-YP335906SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP335906SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP335906SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP335906SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP335906SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

EDE1

Uniprot No.

P34216

Alternative Names

EDE1; BUD15; YBL047C; YBL0501; YBL0520EH domain-containing and endocytosis protein 1; Bud site selection protein 15

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Functions at the internalization step of the clathrin-mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner.

Gene References into Functions

When expressed in yeast, human Eps15 localized to the plasma membrane, where it recruited late-phase CME proteins and supported productive endocytosis, identifying it as an Ede1 functional homologue. PMID: 28057762
Hrr25 kinase and Ede1 cooperate to initiate and stabilize endocytic sites PMID: 25625208
a new and widely applicable tool to generate conditionally phosphorylated proteins to identify those whose dephosphorylation is required for cytokinesis. This approach identifies Aip1, Ede1 and Inn1 as cytokinetic regulators. PMID: 25371407
Results show that oligomerization of Ede1 is essential for its localization and function as an early endocytic scaffold protein. PMID: 25366307
Deubiquitination regulates formation of endocytic sites and stability of the endocytic coat and occurs through Ede1, because permanently ubiquitinated Ede1 phenocopies deletion of UBP2 and UBP7. PMID: 24746795
Ede1 ubiquitin associated domain-ubiquitin interaction reveal several key affinity determinants and, unexpectedly, a negative affinity determinant in the wild-type Ede1 protein PMID: 16563434

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Subcellular Location

Cytoplasm. Note=Localized to actin cortical patches concentrated in the developing bud tip in cells with small buds and at the mother-daughter neck in cells undergoing cytokinesis. Localization can be maintained in the absence of polymerized actin filaments.

Protein Families

VDP/USO1/EDE1 family

Database Links

KEGG: sce:YBL047C

STRING: 4932.YBL047C

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