Recombinant Saccharomyces cerevisiae GTP-binding protein YPT1 (YPT1)

Code CSB-YP360492SVG
Size US$436
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
YPT1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
YPT1; YP2; YFL038C; GTP-binding protein YPT1; Protein YP2; Rab GTPase YPT1; Transport GTPase YPT1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Source
Yeast
Expression Region
1-206aa
Target Protein Sequence
MNSEYDYLFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESFNGVKMWLQEIDRYATSTVLKLLVGNKCDLKDKRVVEYDVAKEFADANKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLNETTQKKEDKGNVNLKGQSLTNTGGGCC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
25.2kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. YPT1 regulates the trafficking of secretory vesicles from the endoplasmic reticulum (ER) to the Golgi. Vesicular transport depends on shuttling of YPT1 between membrane and cytosol by GDI1, probably by recycling it to its membrane of origin after a vesicle fusion event. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Also involved in the recycling of membrane proteins.
Gene References into Functions
  1. TRAPPIII and Ypt1 control membrane trafficking events under normal growth conditions and during autophagy. PMID: 29109089
  2. Since these there proteinases belong to the CPY pathway, YPT1 is even believed to up-regulate this trafficking pathway in yeast cells. Future studies, however, should be carried out to discover the mechanisms that allow YPT1 to recruit these proteins into yeast vacuoles PMID: 26895320
  3. that changes in Ypt1 and Ypt31 activity affect Golgi cisternal progression, early-to-transitional and transitional-to-late, respectively PMID: 26906739
  4. Ypt1 binds and activates Hrr25 to spatially regulate its kinase activity. PMID: 26195667
  5. Finally, three sequential steps of this pathway are delineated: Atg9-dependent exit from the ER en route to autophagy, Ypt1- and core Atgs-mediated pre-autophagsomal-structure organization, and Ypt51-mediated delivery of APs to the vacuole PMID: 26181331
  6. results suggest that Ypt1p is involved in the cellular protection process under heat stress conditions PMID: 26169936
  7. These findings establish a role for an autophagy-specific Ypt1 module in the regulation of ER-phagy. Moreover, because Ypt1 is a known key regulator of ER-to-Golgi transport, these findings establish a second role for Ypt1 at the ER PMID: 23924895
  8. Findings show that Ypt1/Rab1 infindings show that Ypt1/Rab1 interacts with Atg1/Ulk1 in yeast and mammalian cells. PMID: 23716696
  9. Trs85 and Ypt1 are localized to the preautophagosomal structure in an Atg9-dependent manner. PMID: 23129774
  10. Our findings establish that Ypt1 contributes to regulation of UPR signaling dynamics by promoting the decay of HAC1 RNA, suggesting a potential regulatory mechanism for linking vesicle trafficking to the UPR and ER homeostasis PMID: 22844259
  11. Ypt1 and Atg11 colocalize with Trs85, a Ypt1 activator subunit, and together they regulate selective autophagy. PMID: 22509044
  12. Ypt1 is essential for autophagy. PMID: 20375281
  13. alpha-Synuclein expression blocks ER-to-Golgi vesicular trafficking; in a genomewide screen, the largest class of toxicity modifiers were proteins functioning at this step including Ypt1p which associated with cytoplasmic alphaSyn inclusions PMID: 16794039
  14. These results show that Trs65 plays a role in the Ypt guanine nucleotide exchanger activity of TRAPP II in concert with the two other TRAPP II-specific subunits. PMID: 17475775
  15. These results confirm the link between translation and vesicular trafficking and reinforce the implication of eIF5A in protein synthesis. PMID: 18568365
  16. Study presents the structure of a heteropentameric TRAPPI assembly complexed with Ypt1p & leads to proposal for mechanism for guanine nucleotide exchange in which each subunit in the TRAPPI subassembly contributes to the activity of this multimer. PMID: 18585354
  17. identify the kinetic and thermodynamic bases by which TRAPP catalyzes nucleotide exchange from Ypt1p PMID: 19361519
  18. Data show that Gyp1p, a GAP for Ypt1p, specifically interacts with Ypt32p, and that this interaction is important for the localization and stability of Gyp1p. PMID: 19666511

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cytoplasm. Preautophagosomal structure membrane; Lipid-anchor; Cytoplasmic side. Note=ER and Golgi when GTP-bound. Cytoplasmic when bound to GDI1.
Protein Families
Small GTPase superfamily, Rab family
Database Links

KEGG: sce:YFL038C

STRING: 4932.YFL038C

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