Recombinant Saccharomyces cerevisiae Guanine nucleotide-binding protein subunit beta(STE4)

Code CSB-YP323058SVG
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Source Yeast
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Code CSB-EP323058SVG
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Source E.coli
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Code CSB-EP323058SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323058SVG
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Source Baculovirus
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Code CSB-MP323058SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names STE4
Uniprot No. P18851
Alternative Names STE4; YOR212W; YOR50-2Guanine nucleotide-binding protein subunit beta
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region 1-423
Target Protein Sequence MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH AQINKAKHKI QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW SRDSKRILSA SQDGFMLIWD SASGLKQNAI PLDSQWVLSC AISPSSTLVA SAGLNNNCTI YRVSKENRVA QNVASIFKGH TCYISDIEFT DNAHILTASG DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS NTFASCGSDG YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ GVVSLDFSAS GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS PDGLAVCTGS WDSTMKIWSP GYQ
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Implicated in the a- and alpha-factor response pathway. The beta and gamma chains of the putative yeast mating response pathway G protein play a positive role in initiation of the mating response. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Gene References into Functions
  1. The Dse1-Gbeta interaction antagonizes Gbetagamma signaling, including pheromone-activation of the invasive growth pathway. PMID: 19820940
  2. KSS1 is activated by Ste4. PMID: 15192700
Protein Families WD repeat G protein beta family
Database Links

KEGG: sce:YOR212W

STRING: 4932.YOR212W

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