Product Details

Purity

>85% (SDS-PAGE)

Target Names

ASC1

Uniprot No.

P38011

Alternative Names

ASC1; CPC2; YMR116C; YM9718.15C; Guanine nucleotide-binding protein subunit beta-like protein; Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1; Small ribosomal subunit protein RACK1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

2-319

Target Protein Sequence

ASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTLR LWDVATGETY QRFVGHKSDV MSVDIDKKAS MIISGSRDKT IKVWTIKGQC LATLLGHNDW VSQVRVVPNE KADDDSVTII SAGNDKMVKA WNLNQFQIEA DFIGHNSNIN TLTASPDGTL IASAGKDGEI MLWNLAAKKA MYTLSAQDEV FSLAFSPNRY WLAAATATGI KVFSLDPQYL VDDLRPEFAG YSKAAEPHAV SLAWSADGQT LFAGYTDNVI RVWQVMTAN

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, RACK1 serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Involved in the negative regulation of translation of a specific subset of proteins.

Gene References into Functions

Data provide evidence that Asc1 seems to connect signal transduction not only with the translational machinery, but also with fundamental nuclear processes. PMID: 28982715
These indicate that ribosome-associated Asc1/RACK1 facilitates endonucleolytic cleavage of nonstop mRNA by stalled ribosomes and represses the levels of aberrant products even in the absence of Dom34. We propose that Asc1/RACK1 acts as a fail-safe in quality control for nonstop mRNA. PMID: 27312062
identify Asc1p (aka RACK1) and Rps3p, both stable 40S subunit components, as additional 18S nonfunctional 18S rRNA decay factors PMID: 28956756
It is the paper where ASC1 gene is identified and cloned and named. The effect of ASC1 deletion is also studied. PMID: 9504906
Translation fidelity depends upon Asc1-mediated quality control. PMID: 25792604
These results indicate the intertwined structure of a relatively small and stable protein core and the remaining regions of different levels of flexibility. PMID: 24591271
Genetic interactions between dys1-1 mutants and mutant PKC1 and ASC1, implicate a role for HYP2 (eIF5A homolog) and Asc1 in the maintenance of cell integrity at the translational level. PMID: 23573236
Evidence of the translational regulation of such transcription factors suggests that ribosomal Asc1p is involved in signal transduction pathways and controls the biosynthesis of the respective final transcriptional regulators. PMID: 23071099
The structure reveals an atypical mode of dimerization where monomers intertwine on blade 4, thus exposing a novel surface of the protein to potential interacting partners. PMID: 21704636
Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast PMID: 17591772
RACK1 functions from the ribosome, implying a physical link between the eukaryotic ribosome and cell signaling pathways in vivo. PMID: 19114558

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Subcellular Location

Cytoplasm.

Protein Families

WD repeat G protein beta family, Ribosomal protein RACK1 subfamily

Database Links

KEGG: sce:YMR116C

STRING: 4932.YMR116C

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Code	CSB-EP339901SVG-B
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Code	CSB-MP339901SVG
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Source	Mammalian cell
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Recombinant Saccharomyces cerevisiae Guanine nucleotide-binding protein subunit beta-like protein (ASC1)

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