Recombinant Saccharomyces cerevisiae HNRNP arginine N-methyltransferase (HMT1)

Code CSB-YP339910SVG
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Source Yeast
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Code CSB-EP339910SVG
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Source E.coli
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Code CSB-EP339910SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339910SVG
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Source Baculovirus
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Code CSB-MP339910SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HMT1
Uniprot No.
Alternative Names
HMT1; ODP1; RMT1; YBR034C; YBR0320; Protein arginine N-methyltransferase 1; EC 2.1.1.-; Major type I protein arginine N-methyltransferase; Type I PRMT; hnRNP arginine N-methyltransferase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-348
Target Protein Sequence
MSKTAVKDSA TEKTKLSESE QHYFNSYDHY GIHEEMLQDT VRTLSYRNAI IQNKDLFKDK IVLDVGCGTG ILSMFAAKHG AKHVIGVDMS SIIEMAKELV ELNGFSDKIT LLRGKLEDVH LPFPKVDIII SEWMGYFLLY ESMMDTVLYA RDHYLVEGGL IFPDKCSIHL AGLEDSQYKD EKLNYWQDVY GFDYSPFVPL VLHEPIVDTV ERNNVNTTSD KLIEFDLNTV KISDLAFKSN FKLTAKRQDM INGIVTWFDI VFPAPKGKRP VEFSTGPHAP YTHWKQTIFY FPDDLDAETG DTIEGELVCS PNEKNNRDLN IKISYKFESN GIDGNSRSRK NEGSYLMH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that catalyzes both the mono- and asymmetric (type I) dimethylation of the guanidino nitrogens of arginine residues in a variety of RNA-binding proteins such as heterogeneous nuclear ribonucleoproteins (hnRNPs) and small nuclear ribonucleoproteins (snRNPs). Methylates NAB2, NPL3, HRP1 and YRA1, shuttling hnRNPs involved in mRNA processing and export, facilitating their export out of the nucleus. Methylation of NPL3 weakens its interaction with THO2, a component of the TREX (transcription/export) complex important for transcriptional elongation and recruitment of mRNA export factors. Methylates the hnRNP HRB1, but does not influence its subcellular location. Methylates the nucleolar proteins GAR1, NOP1 and NSR1. Methylates the snRNP SNP1 and modulates the cotranscriptional recruitment of splicing factors. Dimethylates free histone H4 (HHF1/HHF2) at 'Arg-4' (H4R3me2a) and plays a role in preservation and establishment of silent chromatin domains. Mono- and dimethylates ribosomal protein S2 (RPS2) at 'Arg-11'. Methylates the catalytic subunit of the SWI/SNF chromatin-remodeling complex SNF2.
Gene References into Functions
  1. Data suggest that Hmt1-based arginine methylation is required for Scd6 localization and function. PMID: 27776129
  2. Arginine methylation is a widespread modification, with Hmt1 being the major arginine methyltransferase in S. cerevisiae. PMID: 26572822
  3. the methyltransferase activity of Hmt1 on histone H3R2 requires reciprocal contributions from two Hmt1 molecules, suggesting an intermolecular trans-complementary mechanism by which Hmt1 dimer methylates its substrates PMID: 25715670
  4. Carried out a proteomic profiling experiment to comprehensively identify the physical interactors of Hmt1, the budding yeast homolog for human PRMT1. Snf2 was experimentally confirmed as a robust substrate of Hmt1 in vitro. PMID: 22997150
  5. Interactions between HMT1 and genes encoding components of the histone deacetylase complex Rpd3L, are reported. PMID: 22953000
  6. These results are suggestive of a role for Rmt1 in modifying the function of Rps2 in a manner distinct from that occurring in S. pombe and mammalian cells. PMID: 20035717
  7. Loss of Hmt1's catalytic activity results in dimethylated Arg-3 histone H4 occupancy across silent chromatin regions. PMID: 17158743
  8. Results reveal a functional connection between histone acetylation, methylation, and two of the responsible enzymes, Gcn5p and Hmt1p. PMID: 19358899

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Subcellular Location
Nucleus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
Database Links

KEGG: sce:YBR034C

STRING: 4932.YBR034C

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