Recombinant Saccharomyces cerevisiae Inhibitory regulator protein IRA2(IRA2) ,partial

Code CSB-YP322501SVG
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Source Yeast
Code CSB-EP322501SVG
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Source E.coli
Code CSB-EP322501SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP322501SVG
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Source Baculovirus
Code CSB-MP322501SVG
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names IRA2
Uniprot No. P19158
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region 1-3079
Target Protein Sequence MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV EEYSSFISCR SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD VIESILIILR LLSDALEYNW QNQESLHYND ISTHVEHDQE QKYRPKLNSI LPDYSSTHSN GNKHFFHQSK PQALIPELAS KLLESCAKLK FNTRTLQILQ NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS TLGHILRFVA ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY YELFNLLKDN NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS SPSSSSPSSP PSSSSSDNNN QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG EYDPSLPDTP TMSNITISAS SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA EQQSRASYDA HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI TQGLKKLTSL PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF TMTSAVFLVD RNLPSVLFAK RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK NSTTFVQLQL IFFSSAIQFD HELLLARLSI DTMANNLNMQ KLCLYTEGFR IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY FDEQITDIVA SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL GGPSSIIRNK IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS AAFGIRSPLQ KIRTRRYSDE SLGKFMKSTN NYIQEHLIPK DLNEATLQDA RRIMINIFSI FKRPNSYFII PHNINSNLQW VSQDFRNIMK PIFVAIVSPD VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT VTLFAMGLFD LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN IEFVEAMCHD NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW HTYTHSKSLE KQERNDFRNF AGILASLSGI LFINKKILQE MYPYLLDTVS ELKKNIDSFI SKQCQWLNYP DLLTRENSRD ILSVELHPLS FNLLFNNLRL KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY VMMLFSTEIV DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS TAIAYLTRHT FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN YPVFLRHKMS VLNDNVILSL TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF LEVFINIVTN YRTYTAKTDL GKLEAADKFL RYTIEHPQLS SFGAAVCPAS DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI LRRNSCATRS LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF LRFFCPALVS PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA LCSQKDFLKE CSDRIFRFLA ELCRTDRTID IQVRTDPTPI AFDYQFLHSF VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK TTFLLIDDVL GQLGQPKMEF SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV HESTSSEGIP IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK IPHYFINSNS DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP VSLKIGDIYF QVLHETPRQY KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI TGVAAEFTVT FQDERRLIFS SPKYLEIVKM FYYAQIRLES EYEMDNNSST SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS SYNLIAATEA SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI RLSVRETDFK AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK KTISLLTVLP TTEVANNIIQ KILAKIRSFL PSLKLEAMTQ SWSELTILVK ISIHVFFETS LLVQMYLPEI LFIVSLLIDV GPRELRSSLH QLLMNVCHSL AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL QIFSASSFAS KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK IVEGLDPNLD LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF ENESETSLIS TLLKGRKFAH TFLSKIENLS GIVWNEDNFT HILIFIINKG LSNPFIKSTA FDFLKMMFRN SYFEHQINQK SDHYLCYMFL LYFVLNCNQF EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT LYQGAILFKC SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM MIPLDVKPED IYERKRIMTM ILSRMSCSA
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.
Subcellular Location Cytoplasm
Database Links

KEGG: sce:YOL081W

STRING: 4932.YOL081W

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