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Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8 (CIN8), partial

Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8 (CIN8), partial

Code	CSB-YP338877SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP338877SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP338877SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP338877SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP338877SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CIN8

Uniprot No.

P27895

Alternative Names

CIN8; KSL2; YEL061CKinesin-like protein CIN8; Chromosome instability protein 8

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3.

Gene References into Functions

Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement PMID: 29117528
Data suggest that approximately four Cin8 motor domains can bind each alpha/beta-tubulin dimer within microtubule lattice; Cin8 binds microtubules not only at the canonical site, but also one or more separate "non-canonical" sites. PMID: 28701465
The tail domain is one of the factors that regulate Cin8 directionality. PMID: 25991727
The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8. PMID: 22101328
Dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase. PMID: 21378308
Cin8 can switch between two states of directional motility. On individual microtubules, Cin8 motors move toward the minus end, they switch to plus-end movement when part of a team of mechanically coupled motors cross-linking antiparallel microtubules. PMID: 21350123
Data suggest that spindle disassembly is achieved by mechanistically distinct but functionally overlapping subprocesses, which are largely governed by the anaphase-promoting complex, Aurora B kinase, and kinesin-8. PMID: 21079246
Results suggest that binding of microtubules is not sufficient for the functionality of Cin8p and that microtubule cross-linking by the tetrameric complex is essential for Cin8p mitotic functions. PMID: 16829678
Spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor protein Cin8 PMID: 18628309
Data observed that deletion of Cin8, a microtubule-crosslinking kinesin-5 motor protein, reduced the recovery rate in anaphase spindles, indicating that Cin8 contributes to the destabilization of iMT plus-ends. PMID: 19270715