Recombinant Saccharomyces cerevisiae Lipid droplet hydrolase 1 (LDH1)

Code CSB-YP336406SVG
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Source Yeast
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Code CSB-EP336406SVG
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Source E.coli
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Code CSB-EP336406SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336406SVG
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Source Baculovirus
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Code CSB-MP336406SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LDH1
Uniprot No.
Alternative Names
LDH1; YBR204C; YBR1444; Lipid droplet hydrolase 1; EC 3.1.1.-; Lipid esterase; EC 3.1.1.-; Triacylglycerol lipase; EC 3.1.1.3
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-375
Target Protein Sequence
MNMAERAEAT KSWSCEPLSG KTLEEIVQNA ENAADLVAYI RKPEVDLDFR LKFIAEHEEF FNVQLSDRNS RIRTCHNLSD KGIRGDTVFV FVPGLAGNLE QFEPLLELVD SDQKAFLTLD LPGFGHSSEW SDYPMLKVVE LIFVLVCDVL RKWSTAVPNN DNVNPFNGHK IVLVGHSMGC FLACHLYEQH MADTKAVQTL VLLTPPKAHI EQLSKDKHII QWALYGVFKL PWLFDVYRNK FDQVKGLQSS GIKQYFYQQG DDVKLKYRKF WQFKNNISNK SRTIIGYLLG WETVDWVKFN GVLTQTDMKQ KIIIFGAEKD PIAPIENLEF YKQTINKECL RKVIILPDCS HNLCFDRPEL VCENFQREVI DNSKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity.
Gene References into Functions
  1. Ldh1p is not required for the function and biogenesis of peroxisomes, and targeting of Ldh1p to lipid droplets occurs independently of the PTS1 receptor Pex5p. [Ldh1p] PMID: 21478430
  2. Ldh1p is thought to play a role in maintaining the lipid homeostasis in yeast by regulating both phospholipid and nonpolar lipid levels PMID: 21478434
Subcellular Location
Lipid droplet.
Protein Families
AB hydrolase superfamily, Lipase family
Database Links

KEGG: sce:YBR204C

STRING: 4932.YBR204C

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