Recombinant Saccharomyces cerevisiae Meiosis-specific protein HOP1(HOP1) ,partial

Code CSB-YP323181SVG
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Source Yeast
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Code CSB-EP323181SVG
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Source E.coli
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Code CSB-EP323181SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323181SVG
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Source Baculovirus
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Code CSB-MP323181SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names HOP1
Uniprot No. P20050
Alternative Names HOP1; YIL072W; Meiosis-specific protein HOP1
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Probable constituent of the synaptonemal complex during meiosis. May interact with RED1.
Gene References into Functions
  1. Hop1 phospho-T318 and phospho-S298 constitute key components of the Tel1/Mec1- based meiotic recombination surveillance (MRS) network and facilitate coupling of meiotic recombination and progression during both unperturbed and challenged meiosis PMID: 26225562
  2. N-terminal domain of Hop1 protein is dispensable for DNA binding, bridging, and synapsis of double-stranded DNA molecules but is necessary for spore formation. PMID: 23841450
  3. Hop1 protein exhibits the ability to bridge noncontiguous DNA segments into intramolecular stem-loop structures in which the DNA segments appear to be fully synapsed within the filamentous protein stems PMID: 23121004
  4. Xrs2 may enable Pch2 to remodel chromosome structure adjacent to the site of a DSB and thereby promote accessibility of Hop1 to the Tel1 kinase PMID: 22072981
  5. Hop1 protein might coordinate the physical monitoring of meiotic recombination intermediates with the process of branch migration of Holliday junction. PMID: 17027027
  6. Hop1 ZnF motif plays a key role in the physical monitoring of recombination intermediates and branch migration of the Holliday junction. PMID: 17935355
  7. Hyperabundance Along Pachytene Chromosomes; Differential Hop1/Zip1 Hyperabundance Depends on Pch2 PMID: 18305165

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Subcellular Location Nucleus, Chromosome
Database Links

KEGG: sce:YIL072W

STRING: 4932.YIL072W

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