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Recombinant Saccharomyces cerevisiae Minichromosome maintenance protein 5 (MCM5), partial

Recombinant Saccharomyces cerevisiae Minichromosome maintenance protein 5 (MCM5), partial

Code	CSB-YP341072SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP341072SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP341072SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP341072SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP341072SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MCM5

Uniprot No.

P29496

Alternative Names

MCM5; CDC46; YLR274W; L9328.1; Minichromosome maintenance protein 5; EC 3.6.4.12; Cell division control protein 46

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.

Gene References into Functions

The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate. PMID: 20484375
A domain-push model is proposed to explain the bypass activity of mutant proline62leucine BOB1, which has a major effect on helicase activity, but no significant impact on DNA binding or ATPase of Methanobacterium thermoautotrophicum MCM gene. PMID: 16401076
Mcm5 protein has a positive role in origin binding, which requires coordination by all six Mcm2-7 subunits in the hexamer. PMID: 18660534
Conclusions predict a structural discontinuity between Mcm2 and Mcm5 and demonstrate that in contrast to other hexameric helicases, the six Mcm2-7 active sites are functionally distinct. PMID: 18662997
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA. PMID: 19896182

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Subcellular Location

Nucleus. Note=Mobilized from the cytoplasm to the nucleus as mitosis is completed. Remains in the nucleus until the initiation of the next round of replication.

Protein Families

MCM family

Database Links

KEGG: sce:YLR274W

STRING: 4932.YLR274W

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