Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM12 (TIM12)

Code CSB-YP333739SVG
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Source Yeast
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Code CSB-EP333739SVG
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Source E.coli
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Code CSB-EP333739SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333739SVG
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Source Baculovirus
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Code CSB-MP333739SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIM12
Uniprot No.
Alternative Names
TIM12; MRS5; YBR091C; YBR0812; Mitochondrial import inner membrane translocase subunit TIM12; Mitochondrial regulator of splicing 5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-109
Target Protein Sequence
SFFLNSLRG NQEVSQEKLD VAGVQFDAMC STFNNILSTC LEKCIPHEGF GEPDLTKGEQ CCIDRCVAKM HYSNRLIGGF VQTRGFGPEN QLRHYSRFVA KEIADDSKK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as external driving force. In the TIM22 complex, it acts as a docking point for the soluble TIM9-TIM10 heterohexamer that guides the target proteins in transit through the aqueous mitochondrial intermembrane space.
Gene References into Functions
  1. study found that TIM12 forms dimers that bind to Tim9; in this interaction, Tim12 undergoes structural changes that may be important for transport of its substrates in the mitochondrial carrier import pathway PMID: 17627602
  2. the essential role of Tim12 relies on its unique assembly properties that allow this subunit to bridge the soluble and membrane-embedded translocases in the carrier import pathway. PMID: 18387953
  3. Data show that assembly of the three small Tim proteins, Tim9, -10, and -12, precedes docking to the mitochondrial carrier translocase. PMID: 18421298
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion intermembrane space.
Protein Families
Small Tim family
Database Links

KEGG: sce:YBR091C

STRING: 4932.YBR091C

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