Recombinant Saccharomyces cerevisiae Mitotic spindle checkpoint component MAD2 (MAD2)

Code CSB-YP340327SVG
MSDS
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Source Yeast
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Code CSB-EP340327SVG
MSDS
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Source E.coli
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Code CSB-EP340327SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340327SVG
MSDS
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Source Baculovirus
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Code CSB-MP340327SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAD2
Uniprot No.
Alternative Names
MAD2; YJL030W; J1256Mitotic spindle checkpoint component MAD2; Mitotic MAD2 protein
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-196
Target Protein Sequence
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.
Gene References into Functions
  1. Mad2 recruitment to the kinetochore and it's role in the spindle assembly checkpoint PMID: 27280788
  2. the very same dimerization interface of Mad2 is required for two different, yet fundamental, consecutive steps of SAC signaling: at the kinetochores for priming Cdc20/Mad2 binding, away from kinetochores for stabilizing this complex PMID: 23000150
  3. Mad2 is an important meiotic cell cycle regulator that ensures the timely degradation of anaphase-promoting complex substrates and the proper orchestration of the meiotic divisions PMID: 21697504
  4. Mad2 prolongs DNA damage checkpoint arrest caused by a double-strand break via a centromere-dependent mechanism. PMID: 20096585
  5. Data demonstrate that Mad2p localization at nuclear pore complexes is sensitive to nuclear levels of Ran and suggest that release of Mad2p from NPCs is closely linked with spindle assembly checkpoint activation in yeast. PMID: 15701789
  6. Our biochemical and genetic evidence shows that the interaction of open and closed Mad2 is essential for the spindle assembly checkpoint and is conserved in evolution. PMID: 16818718
  7. results surpass previously formulated objections to the Mad2-template model and predict that the release of Mad2 from Cdc20 is an energy-driven process PMID: 19143472

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Subcellular Location
Nucleus.
Protein Families
MAD2 family
Database Links

KEGG: sce:YJL030W

STRING: 4932.YJL030W

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