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Recombinant Saccharomyces cerevisiae Pachytene checkpoint protein 2 (PCH2), partial

Recombinant Saccharomyces cerevisiae Pachytene checkpoint protein 2 (PCH2), partial

Code	CSB-YP339918SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP339918SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP339918SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP339918SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP339918SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PCH2

Uniprot No.

P38126

Alternative Names

PCH2; YBR186W; YBR1308; Pachytene checkpoint protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region.

Gene References into Functions

pch2Delta showed approximately 50% genome-wide increase in crossover frequency, elevated noncrossovers and loss of chromosome size dependent double-strand break formation. PMID: 28315832
discuss our current understanding of the function of Pch2(TRIP13) during meiotic and mitotic cell divisions, with a focus on its enzymatic role towards HORMA domain-containing clients PMID: 25895724
Pch2 displaces Hop1 from large DNA substrates and both ATP binding and hydrolysis by Pch2 are required for Pch2-Hop1 transactions PMID: 24367111
Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. PMID: 21515575
tgs1Delta cells are specifically defective in splicing PCH2 meiotic pre-mRNAs. PMID: 21398639
PCH2 is required for recombination and normal higher-order chromosome structure during meiosis. PMID: 20711356
during WT meiosis, Pch2 promotes progression of SC-associated CO and NCO recombination complexes at a regulated early-midpachytene transition that is rate-limiting for later events PMID: 18305165
Roles of Pch2 in controlling both chromosome axis morphogenesis and crossover placement suggest linkage between these processes. PMID: 19629172
Study provides four evidence suggesting that PCH2 regulates the formation and distribution of crossover events required to promote chromosome segregation at Meiosis I. PMID: 19629178

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Subcellular Location

Nucleus, nucleolus. Chromosome. Note=The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent.

Protein Families

AAA ATPase family, PCH2 subfamily

Database Links

KEGG: sce:YBR186W

STRING: 4932.YBR186W

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