Recombinant Saccharomyces cerevisiae Protein MSS51, mitochondrial (MSS51)

Code CSB-YP341308SVG
MSDS
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Source Yeast
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Code CSB-EP341308SVG
MSDS
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Source E.coli
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Code CSB-EP341308SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341308SVG
MSDS
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Source Baculovirus
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Code CSB-MP341308SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MSS51
Uniprot No.
Alternative Names
MSS51; YLR203C; L8167.17; Protein MSS51; mitochondrial; Mitochondrial splicing suppressor protein 51
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
27-436
Target Protein Sequence
VVSH QTRRHLMGFV RNALGLDPPP SPEDPTPENR FHPWDQSPSV DLRERAAKIR TLAHCPVTGK DINYTCPLSG IPTHHSREAW EMDKAYHDSK KYEILKKVNI YEHDLRSGRP FPEFDFPQQQ GYDKAVNLTN WDLFFYTRSF YSMDTEFQLA AVTKMLSYPI TIGSLLHKFS PYSLNPKGPI TLEGLKSLAA LRYTLYPLEN RSLPTTTKNR AMRIFILGAR AEAQLPGHVW KQLQFLFPEQ SFEIHFIGPE CLYKRDKQEY VKSTTPVVQR VDETLKFIYR TNFFEVFHEA QDFFPYDPYM DVFFTFHPGY ASPESHGSWM GETMKALLET KCAIFTTGFN KKDLTDDINL VKSKYGKEMD VLMEPVRNVF GSTKWELNDM NPQEVYQFNM YIAGFRGKRY HTIKRQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has a dual role in the assembly of cytochrome oxidase subunit 1 (COX1). It has a regulative function on COX1 synthesis, acting as a translational activator specific for the COX1 mRNA, and it also binds to newly synthesized COX1 protein. Together with COX14, may act as a chaperone that binds efficiently unassembled COX1 and prevents it from unproductive aggregation. When bound to COX1, MSS51 is unable to interact with the COX1 mRNA and translation is halted. This may be a feedback control that ensures that COX1 is only produced as long as potentially harmful assembly intermediates are not accumulating.
Gene References into Functions
  1. The physical interaction OF Mss51 & Cox14 depends on Cox1 synthesis, showing dynamic assembly of early cytochrome c oxidase intermediates nucleated by Cox1. PMID: 19710419
  2. Ssc1, by interacting with Mss51 and Mss51-containing complexes, plays a critical role in Cox1 biogenesis, COX assembly, and the translational regulation of these processes. PMID: 19858289
  3. These results support a key role for Mss51p in translationally regulating Cox1p synthesis by the status of cytochrome oxidase assembly. PMID: 17135289
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
MSS51 family
Database Links

KEGG: sce:YLR203C

STRING: 4932.YLR203C

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