Recombinant Saccharomyces cerevisiae Replication factor A protein 1(RFA1) ,partial

Code CSB-YP322138SVG
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Source Yeast
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Code CSB-EP322138SVG
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Source E.coli
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Code CSB-EP322138SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322138SVG
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Source Baculovirus
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Code CSB-MP322138SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names RFA1
Uniprot No. P22336
Alternative Names RFA1; BUF2; RPA1; YAR007C; FUN3; Replication factor A protein 1; RF-A protein 1; DNA-binding protein BUF2; Replication protein A 69 kDa DNA-binding subunit; Single-stranded DNA-binding protein
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Gene References into Functions
  1. Overexpression of Rad51 was also found to suppress the rfa1 BIR defects. We suggest that Rad51 binding to the ssDNA formed by excessive end resection and during D-loop migration can partially compensate for dysfunctional RPA. PMID: 28009302
  2. RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. PMID: 29033322
  3. Force regulated dynamics of RPA1 on a DNA fork during DNA replication has been reported. PMID: 27016742
  4. secondary structures within single-stranded DNA are potent instigators of genome instability, and RPA and Mre11-Sae2 play important roles in preventing their formation and propagation, respectively. PMID: 26545079
  5. RPA could exclude Ku from a partially resected structure with a 22-nt ssDNA tail and thus restored processing by Exo1. However, at a 40-nt tail, Ku remained stably associated at the ssDNA-dsDNA junction, and RPA simultaneously engaged the ssDNA region. PMID: 26067273
  6. DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance PMID: 26253534
  7. the N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks PMID: 22820947
  8. Data indicate that replication protein A (RPA) complex RFA1 is required for DNA end resection by Sgs1-Dna2 and Exo1. PMID: 23706822
  9. A novel pathway of telomere end protection that utilizes a previously unsuspected function of RPA at the telomeres. PMID: 23312805
  10. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. PMID: 22354040
  11. this work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells. PMID: 22194703
  12. Isw2 and Ino80 are both enriched at stalled replication forks and physically and specifically interact with a single-stranded DNA binding protein, replication protein A. PMID: 21930788
  13. Data show that Pif1 overexpression causes relocalization of the DNA damage response factors Rfa1 and Mre11 into nuclear foci and activation of the Rad53 DNA damage checkpoint kinase, indicating that the toxicity is caused by accumulation of DNA damage. PMID: 19704012
  14. RPA appears to play a role in strand invasion as well as in facilitating Rad51 binding to ssDNA, possibly by stabilizing the displaced ssDNA PMID: 14737196
  15. Structure of the DNA-binding domain of the large subunit of replication protein A, a single-stranded DNA (ssDNA)-binding heterotrimeric protein with multiple roles in DNA metabolism. Study of the domain's interactions with ssDNA & SV40 T antigen. [RPA70] PMID: 16043636
  16. These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. PMID: 16678108
  17. Control of telomeric recombination by RFA1. PMID: 17202155
  18. We show that Pol eta is less accurate than other yeast DNA polym and the accessory proteins have little effect on fidelity when bypassing a TT dimer. Accessory proteins participate in pol eta functions, but they do not suppress UV mutagenesis. PMID: 17608453
  19. Rad52 displaces RPA from single-stranded DNA using the acidic domain as a molecular lever PMID: 18703507
  20. Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus. PMID: 19597487

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Subcellular Location Nucleus
Protein Families Replication factor A protein 1 family
Database Links

KEGG: sce:YAR007C

STRING: 4932.YAR007C

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