Recombinant Saccharomyces cerevisiae Replication factor A protein 1 (RFA1), partial

1. Overexpression of Rad51 was also found to suppress the rfa1 BIR defects. We suggest that Rad51 binding to the ssDNA formed by excessive end resection and during D-loop migration can partially compensate for dysfunctional RPA. PMID: 28009302
2. RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. PMID: 29033322
3. Force regulated dynamics of RPA1 on a DNA fork during DNA replication has been reported. PMID: 27016742
4. secondary structures within single-stranded DNA are potent instigators of genome instability, and RPA and Mre11-Sae2 play important roles in preventing their formation and propagation, respectively. PMID: 26545079
5. RPA could exclude Ku from a partially resected structure with a 22-nt ssDNA tail and thus restored processing by Exo1. However, at a 40-nt tail, Ku remained stably associated at the ssDNA-dsDNA junction, and RPA simultaneously engaged the ssDNA region. PMID: 26067273
6. DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance PMID: 26253534
7. the N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks PMID: 22820947
8. Data indicate that replication protein A (RPA) complex RFA1 is required for DNA end resection by Sgs1-Dna2 and Exo1. PMID: 23706822
9. A novel pathway of telomere end protection that utilizes a previously unsuspected function of RPA at the telomeres. PMID: 23312805
10. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. PMID: 22354040
11. this work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells. PMID: 22194703
12. Isw2 and Ino80 are both enriched at stalled replication forks and physically and specifically interact with a single-stranded DNA binding protein, replication protein A. PMID: 21930788
13. Data show that Pif1 overexpression causes relocalization of the DNA damage response factors Rfa1 and Mre11 into nuclear foci and activation of the Rad53 DNA damage checkpoint kinase, indicating that the toxicity is caused by accumulation of DNA damage. PMID: 19704012
14. RPA appears to play a role in strand invasion as well as in facilitating Rad51 binding to ssDNA, possibly by stabilizing the displaced ssDNA PMID: 14737196
15. Structure of the DNA-binding domain of the large subunit of replication protein A, a single-stranded DNA (ssDNA)-binding heterotrimeric protein with multiple roles in DNA metabolism. Study of the domain's interactions with ssDNA & SV40 T antigen. [RPA70] PMID: 16043636
16. These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. PMID: 16678108
17. Control of telomeric recombination by RFA1. PMID: 17202155
18. We show that Pol eta is less accurate than other yeast DNA polym and the accessory proteins have little effect on fidelity when bypassing a TT dimer. Accessory proteins participate in pol eta functions, but they do not suppress UV mutagenesis. PMID: 17608453
19. Rad52 displaces RPA from single-stranded DNA using the acidic domain as a molecular lever PMID: 18703507
20. Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus. PMID: 19597487

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KEGG: sce:YAR007C

STRING: 4932.YAR007C