Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5 (CLB5)

Code CSB-YP341136SVG
MSDS
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Source Yeast
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Code CSB-EP341136SVG
MSDS
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Source E.coli
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Code CSB-EP341136SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341136SVG
MSDS
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Source Baculovirus
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Code CSB-MP341136SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLB5
Uniprot No.
Alternative Names
CLB5; YPR120C; P9642.8S-phase entry cyclin-5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-435
Target Protein Sequence
MGENHDHEQS IKRNSMIYNE NERQLCNSNL KILQNKRALS KNDSSSKQQV QDSKPRRALT DVPVNNNPLS QNKRIVAGSK AAKVRREENI RPIVSAVQKR QIYNDRTAAE QEEEEEEEGE DDDAASIVNK KRRIDAEGVS EIVGWQDLDY VEKDDTAMVA EYSAEIFAFL YRRELETLPS HNYLLDKTSK YYLRPSMRTI LVDWLVEVHE KFQCYPETLF LSINLMDRFL AKNKVTMNKL QLLAVTSLFI AAKFEEVNLP KLAEYAYITD GAASKNDIKN AEMFMLTSLE FNIGWPNPLN FLRRISKADD YDPVNRNIGK FILEYAYCCH QFIHLPPSTV SAMAMYIARR MTNRNKNELW NGTLQHYSGG IDPIHDEAFQ SLCIDLVKDI ASSKTHLDSL ILKYKKPRYG SVYFQTFKWC TSEMHSNFQN LFNLK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for efficient progression through S phase and possibly for the normal progression through meiosis. Interacts with CDC28.
Gene References into Functions
  1. Results suggest that Clb5 has a unique ability to trigger premeiotic S phase and that the amino-terminal region of Clb5 contributes to its specificity and regulates the functions performed by the cyclin-Cdk complex. PMID: 22209902
  2. The lack of Snf1 alpha-catalytic subunit down-regulates CLB5 expression. PMID: 20505334
  3. In the presence of replication stress, the unstable Clb5 pool is stabilized, and such stabilization requires neither MBF transcriptional activity nor de novo protein synthesis. PMID: 20538605
  4. The 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from individual origins. PMID: 15611618
  5. Activation of CLB5 during meiosis requires Clb5 activity, suggesting that CLB5 may be regulated by a positive feedback mechanism. PMID: 15654101
  6. Absence of the B-type cyclin Clb5 leads to a delay of premeiotic S phase by separate mechanisms. PMID: 16254243
  7. Clb5 is stable until mitosis PMID: 16508019
  8. Phosphorylation of Fin1 from S phase to metaphase, by the cyclin-dependent kinase Clb5-Cdk1, inhibits Fin1 association with the spindle. In anaphase, when Clb5-Cdk1 is inactivated, Fin1 is dephosphorylated by the phosphatase Cdc14. PMID: 17173039
  9. Clb5 proteolysis involves two different modes for targeting of Clb5 to the proteasome, an APC/C-dependent and an APC/C-independent mechanism. PMID: 17620341
  10. The CLB5 promoter and have identified two middle sporulation elements (MSEs) that act as binding sites for Ndt80 and both MSE display differential effectiveness in their ability to function as cis-acting regulatory sequences in vivo. PMID: 18327887
  11. The coincident destruction of securin, Clb5, and Dbf4 coordinates bulk chromosome segregation with segregation of ribosomal DNA. PMID: 18591250
  12. The temporal program of chromosome replication: genomewide replication in clb5{Delta} Saccharomyces cerevisiae PMID: 18832352

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Protein Families
Cyclin family, Cyclin AB subfamily
Database Links

KEGG: sce:YPR120C

STRING: 4932.YPR120C

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