Product Details

Purity

>85% (SDS-PAGE)

Target Names

GCN2

Uniprot No.

P15442

Alternative Names

GCN2; AAS1; YDR283C; eIF-2-alpha kinase GCN2; EC 2.7.11.1; General control non-derepressible protein 2; Serine/threonine-protein kinase GCN2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on 'Ser-52' in response to low amino acid, carbon, or purine availability. Plays a role as an activator of the general amino acid control (GAAC) pathway required for adapatation to nutrient starvation. Converts phosphorylated eIF-2-alpha/SUI2 either to a competitive inhibitor of translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator GCN4, and hence allowing GCN4-mediated reprogramming of transcription to alleviate nutrient depletion. Binds uncharged tRNAs.; (orthologuous-specific) Binds to aminoacylated tRNA(Phe) less tightly than to deacylated tRNA(Phe). Binds to double-stranded RNA.

Gene References into Functions

findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation PMID: 28057755
GCN2 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. Gcn2 functions upstream of Ure2. PMID: 28007891
Mutations leading to amino acid substitutions on this surface were identified that activate Gcn2 at low levels of tRNA binding (Gcd- phenotype), while other substitutions block kinase activation (Gcn- phenotype PMID: 25695491
To mediate the activation of protein kinase Gcn2, Gcn1 interacts with Rps10. PMID: 25437641
Gcd- substitutions enhance YKD-KD interactions in vitro, whereas Gcn- substitutions in alphaE and alphaI suppress both this effect and the constitutive activation of Gcn2 conferred by YKD Gcd- substitutions PMID: 24811037
Data indicate that the crystal structures of murine and yeast GCN2 protein kinase C-terminal domain (CTD) showed dimers. PMID: 24719324
Activation of Gcn2p by movement protein (MP) expression required a functional Tor1p kinase, since rapamycin treatment alleviated the yeast cell growth defect and blocked eIF2alpha phosphorylation triggered by MP expression PMID: 22087310
the interaction between Pkh1 and Gcn2 was confirmed by co-immunoprecipitation and Pkh1 was shonwn to phosphorylate Gcn2 in vitro. PMID: 22326914
purified eEF1A reduced the ability of Gcn2 to phosphorylate its substrate, eIF2alpha, but did not diminish Gcn2 autophosphorylation. PMID: 21849502
Results highlight the importance of the GCN2 eIF2 kinase pathway for maintaining metabolic homeostasis, contributing to appropriate tRNA charging and growth adaptation in response to culture conditions deficient for tryptophan and arginine. PMID: 20684782
structural analysis of GCN2 autoinhibition and mutational activation PMID: 15964839
the salt bridge interaction and dimer interface observed in the PKR structure is critical for the activity of PKR, GCN2, and PERK PMID: 17202131
Mutationally disrupting the hydrophobic network in various ways constitutively activates eIF2alpha phosphorylation in vivo and bypasses the requirement for a key tRNA binding motif (m2) and Thr-882 in Gcn2. PMID: 19114556
analysis of tRNA charging and activation of the eIF2 kinase Gcn2p PMID: 19546227

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily

Database Links

KEGG: sce:YDR283C

STRING: 4932.YDR283C

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Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase GCN2 (GCN2), partial

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