Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 (RAD53), partial

1. Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands. PMID: 29033319
2. Rad53 can display active conformations and phosphor-priming confers functionally relevant autophosphorylation. PMID: 28858528
3. H2B-mediated Rad53 activation promotes the dissociation of histone chaperone Asf1 from the Rad53 complex. PMID: 25275495
4. Functional relevance of the Rad53 dimer as observed in our crystal structure, and reveal the importance of this mode of head-to-tail, face-to-face dimerization for Rad53 activation. PMID: 24815189
5. Mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo. PMID: 24302356
6. The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DNA double-strand breaks. PMID: 24183725
7. Rad53 regulates a transcriptional response to DNA damage involving more than 600 genes. PMID: 23810556
8. rad53 physical interaction with Dbf4 is required gor Dbf4 phosphorylation for replication checkpoint activation. PMID: 23564203
9. Even in the absence of DNA-damaging agents, the rad53-4AQ mutant, lacking the N-terminal Mec1 phosphorylation site cluster, is synthetic lethal with a deletion of the RAD9 DNA damage checkpoint adaptor. PMID: 23754745
10. The existence of a Rad53p-regulated checkpoint that regulates G1- to S-phase progression in response to loss of mtDNA. PMID: 22927468
11. The role of Rad53 is in degradation of excess cellular histone and in replication initiation are related. PMID: 21099362
12. Threshold levels of Rad53 activity modulate the tolerance to alkylating DNA damage at least by controlling the abundance of the key TLS factor Rev1 bound to chromatin. PMID: 20674515
13. Rad53 is phosphorylated independently of the DNA damage checkpoint response in a cell cycle dependent manner. PMID: 20046099
14. Required for double-strand break-initiated sister chromatin exchange (SCE) and DNA damage-associated SCE after exposure to X-ray-mimetic and ultraviolet-mimetic chemicals. PMID: 16039914
15. A novel role for Rad53 in the initiation of DNA replication that is independent of checkpoint or deoxynucleotide regulation. PMID: 16816422
16. Collectively, the results suggest a novel function of Rad53 in the regulation of polarized cell growth in response to DNA replication stress. PMID: 17130285
17. forkhead-associated domain 1 (FHA1) of Rad53 interacts with a specific threonine of Ptc2, T376 PMID: 17325030
18. Rad53/Chk2 homo-oligomerization is an evolutionarily conserved mechanism that drives Rad53/Chk2 activation and promotes signal amplification in DNA damage responses. PMID: 18239457
19. 3 rad53 alleles in which FHA1 & FHA2 domains were individually or simultaneously transposed to the opposite location. All three mutants were approximately 100-fold hypersensitive to DNA lesions whose survival requires intact Rad53 FHA domain functions. PMID: 18302321
20. distinct phosphatases operate to return Rad53 to its basal state after different genotoxic stresses and a yet unidentified phosphatase may be responsible for the deactivation of Rad53 after replication stress PMID: 18441009
21. These data indicate that Rad53 prevents sister chromatid segregation in the presence of unrepaired programmed meiotic DSBs, thus providing a salvage mechanism ensuring genetic integrity in the gametes even in the absence of the recombination checkpoint. PMID: 18505828
22. Dun1 shows increased affinity for diphosphoyrlated Rad53 cluster domains due to the presence of two separate phosphothreonine-binding pockets. PMID: 18570878

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KEGG: sce:YPL153C

STRING: 4932.YPL153C