Product Details

Purity

>85% (SDS-PAGE)

Target Names

RIM15

Uniprot No.

P43565

Alternative Names

RIM15; TAK1; YFL033C; Serine/threonine-protein kinase RIM15; EC 2.7.11.1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Protein kinase that positively regulates proper entry into stationary phase of cells under nutrient starvation conditions. Involved in glycogen and trehalose accumulation, derepression of stress-induced genes, induction of thermotolerance and starvation resistance, and proper G1 cell cycle arrest. Also involved in the activation of a meiotic genes activation pathway. Phosphorylates IGO1 and IGO2, both involved in the TORC1 control of gene expression and chronological life span.

Gene References into Functions

Rim15 activity increases after cells switch from fermentation to respiration, where Igo1,2 contribute to chromosome maintenance. Interestingly Cln3-Cdk1 also inhibits Rim15 activity, which enables homeostatic control of Whi5 phosphorylation and cell cycle entry. PMID: 28600888
Rim15, a Greatwall-family protein kinase in Saccharomyces cerevisiae, is required for cellular nutrient responses, such as the entry into quiescence and the induction of meiosis and sporulation. (Review) PMID: 28485209
ur data support the hypothesis that metabolic reprogramming to accumulate energy stores and the activation of anti-oxidant defence systems are coordinated by Yak1, Rim15 and Mck1 kinases to ensure quiescence entry and lifespan extension in yeast. PMID: 27923067
Validity of promoter engineering of the RIM15 gene that governs inhibitory control of alcoholic fermentation. PMID: 27633130
G0/G1 transition, via Rim15 and CLN3, might govern the fermentation rate of bottom-fermenting brewer's yeast in high-gravity wort. PMID: 27212268
Rim15 and Sch9 kinases are involved in induction of autophagic degradation of ribosomes in budding yeast PMID: 27659307
A null RIM15 variant confers a greater fermentation rate through the utilization of available glucose instead of its storage. However, this variant has a detrimental effect on fungicide resistance since complex sugars are not synthesized and transported into the membrane. PMID: 26945894
Mpk1 and the greatwall kinase (RIM15) pathway serve TORC1 to coordinate the phosphorylation status of Sic1 and consequently G1-S cell cycle progression with nutrient availability. PMID: 26356805
We propose that the Rim15-Endosulfine( Igo1 and Igo2 )-PP2ACdc55 signalling module triggers entry into quiescence and gametogenesis by regulating dephosphorylation of distinct substrates PMID: 24968058
failure of rim15 cells to adapt to calorie restriction by entering robust post-mitotic state resembles cancer physiology and shows retentostat cultivation of yeast strains can provide relevant models for healthy post-mitotic and transformed human cells PMID: 24487068
We conclude that Greatwall, endosulfines, and PP2A are part of a regulatory module that has been conserved during evolution irrespective of PP2A function in the control of mitosis PMID: 23861665
Rim15 can phosphorylate Msn2, but not Gis1, in vitro, implying different mechanisms for the activation of these transcription factors. PMID: 24140345
Rim15 is required for the biological activity of triclabendazole in the increased resistance to stress. PMID: 23667708
We present data supporting that the PHO pathway functions in parallel to the fermentable growth medium- or Sch9-controlled pathway and that both pathways may share the protein kinase Rim15 in Saccharomyces PMID: 15926040
Rim15 plays a key role in G0 entry through its ability to integrate signaling from the PKA, TORC1, and Pho80-Pho85 pathways PMID: 16308562
These results indicate that the Ras/cAMP/PKA/Rim15/Msn2/4 and the Tor/Sch9/Rim15/Gis1 pathways are major mediators of the calorie restriction-dependent stress resistance and life span extension. PMID: 18225956

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: sce:YFL033C

STRING: 4932.YFL033C

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Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RIM15 (RIM15), partial

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