Recombinant Saccharomyces cerevisiae Spindle assembly checkpoint component MAD1 (MAD1), partial

Code CSB-YP334793SVG
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP334793SVG
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP334793SVG-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP334793SVG
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP334793SVG
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAD1
Uniprot No.
Alternative Names
MAD1; YGL086WSpindle assembly checkpoint component MAD1; Mitotic arrest deficient protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Central component of the spindle assembly checkpoint. Thought to recruit MAD2 to unattached kinetochores. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.
Gene References into Functions
  1. Mad1 recruitment to the kinetochore and it's role in the spindle assembly checkpoint PMID: 27280788
  2. Mad1 is recruited by detached kinetochores competing with alternate other sites in nucleus: Mad1 pool is small enough to be fully depleted by one pair of detached kinetochores. Lateral attachment licenses Mad1 removal from kinetochores after kinetic delay PMID: 26023090
  3. Mad1p senses unattached kinetochores and, by altering nuclear pore complex transport activity, regulates the nuclear environment of the spindle. PMID: 23177738
  4. cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension. PMID: 21593209
  5. Saccharomyces cerevisiae Mad1p C-terminal domain mediates spindle checkpoint function, chromosome transmission fidelity and CEN association PMID: 15802513
  6. Only the C terminus is required for spindle assembly checkpoint (SAC) function and the nuclear pore complex, through Nup53p, may act to regulate the duration of the SAC response. PMID: 16000377

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
MAD1 family
Database Links

KEGG: sce:YGL086W

STRING: 4932.YGL086W

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X