Recombinant Saccharomyces cerevisiae Telomere length regulator protein RIF1 (RIF1), partial

Code CSB-YP333457SVG
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Source Yeast
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Code CSB-EP333457SVG
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Source E.coli
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Code CSB-EP333457SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333457SVG
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Source Baculovirus
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Code CSB-MP333457SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RIF1
Uniprot No.
Alternative Names
RIF1; YBR275C; YBR1743; Telomere length regulator protein RIF1; RAP1-interacting factor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus.
Gene References into Functions
  1. Rif1 SQ/TQ Cluster Domain (SCD) can modulate Rif1 function. As all Rif1 orthologs have one or more SCD domains, these results for yeast Rif1 have implications for the regulation of Rif1 function in humans and other organisms. PMID: 29544213
  2. Engagement of the Rif1 N-terminal domain with telomeres proved essential for checkpoint control and telomere length regulation. Unexpectedly, Rif1 N-terminal domain also promoted NHEJ repair at DNA breaks in yeast, revealing a conserved role of Rif1 in DNA repair. PMID: 28604726
  3. These data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised. Finally, we show that Rif1-Glc7, unlike Sir2, has an important effect on origin firing outside of the rDNA locus that serves to prevent activation of the DNA replication checkpoint PMID: 27820830
  4. Rif1 is phosphorylated by Tel1 during early replication.Tel1 is required for phosphorylation of Rif1 Serine-1308. PMID: 25329891
  5. Data link Rif1 to negative regulation of replication origin firing through recruitment of the Glc7 phosphatase. PMID: 24685139
  6. Findings elucidate the mechanism of action of Rif1 in the control of DNA replication and demonstrate a role of PP1 phosphatases in the regulation of origin firing. PMID: 24656819
  7. Rif1 mechanism regulates origin timing irrespective of proximity to a chromosome end. PMID: 24879017
  8. Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. PMID: 24692507
  9. Rif1 (Rap1-interacting factor 1) controls DNA replication genome-wide and describe how Rif1 opposes DDK function by directing Protein Phosphatase 1 (PP1)-mediated dephosphorylation of the MCM complex PMID: 24532715
  10. Rif1 has important physiological roles in preventing a cell cycle arrest to incipient or small single stranded DNA lesions occurring on chromosomes, particularly on chromosome ends. PMID: 22194703
  11. Saccharomyces cerevisiae Rif1 is an important telomeric factor with an anti-checkpoint role. PMID: 22194704
  12. data supported a model in which Pfa4-dependent palmitoylation of Rif1 anchored it to the inner nuclear membrane, influencing its role in heterochromatin dynamics PMID: 21844336

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Subcellular Location
Nucleus. Chromosome, telomere. Note=Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).
Protein Families
RIF1 family
Database Links

KEGG: sce:YBR275C

STRING: 4932.YBR275C

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