Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 33(VPS33) ,partial

Code CSB-YP322690SVG
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Source Yeast
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Code CSB-EP322690SVG
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Source E.coli
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Code CSB-EP322690SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322690SVG
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Source Baculovirus
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Code CSB-MP322690SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names VPS33
Uniprot No. P20795
Alternative Names VPS33; SLP1; VAM5; YLR396C; L8084.15Vacuolar protein sorting-associated protein 33; Protein SLP1; Vacuolar morphogenesis protein 5
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Essential for vacuolar biogenesis, maturation and function. Involved in the sorting of vacuolar proteins from the Golgi apparatus and their targeting to the vacuole. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.
Gene References into Functions
  1. Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17. PMID: 28925353
  2. Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes. PMID: 25995379
  3. Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes PMID: 24837546
  4. Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition. PMID: 26339030
  5. Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified. PMID: 25564619
  6. we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association PMID: 23051737
  7. contribution of Vps33 to hemifusion and pore opening between vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing. PMID: 20453860
  8. Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane PMID: 15047864

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Subcellular Location Vacuole
Protein Families STXBP/unc-18/SEC1 family
Database Links

KEGG: sce:YLR396C

STRING: 4932.YLR396C

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