Recombinant Saccharopolyspora erythraea Crossover junction endodeoxyribonuclease RuvC(ruvC)

Code CSB-YP389199STB
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Source Yeast
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Code CSB-EP389199STB
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Source E.coli
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Code CSB-EP389199STB-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP389199STB
Size Pls inquire other sizes
Source Baculovirus
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Code CSB-MP389199STB
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ruvC
Uniprot No. A4FBA6
Alternative Names ruvC; SACE_2021Crossover junction endodeoxyribonuclease RuvC; EC 3.1.22.4; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC
Species Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Expression Region 1-199
Target Protein Sequence MRVLGVDPGL TRCGIGVVDG GRGRTVTCVG VGVARTPADQ ELSARLLGVA EAVDEWLDTH RPEVVAIERV FSQHNVRTVM GTAQVSGIVA LAAARRGLPV AFHTPSEVKA AISGSGRADK RQVTTMVTKI LGLAEAPKPA DAADALALAV CHLWRAPMAG RLAEAEAKAA ALARNHKARL KDARQAAAQR AARTGGVQR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
Protein Families RuvC family
Database Links

KEGG: sen:SACE_2021

STRING: 405948.SeryN2_010100027116

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