Recombinant Salmonella paratyphi B Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase(gppA)

Code CSB-YP433958STF
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Source Yeast
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Code CSB-EP433958STF
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Source E.coli
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Code CSB-EP433958STF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP433958STF
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Source Baculovirus
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Code CSB-MP433958STF
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names gppA
Uniprot No. A9MXF2
Alternative Names gppA; SPAB_04859Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase; EC 3.6.1.40; Guanosine pentaphosphate phosphohydrolase; pppGpp-5'-phosphohydrolase
Species Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7)
Expression Region 1-493
Target Protein Sequence MNSTSLYAAI DLGSNSFHML VVREAAGSIQ TLTRIKRKVR LAAGLNNDNH LSAEAMERGW QCLRLFAERL QDIPQPQIRV VATATLRLAV NAGEFIAKAQ TILGCPVQVI SGEEEARLIY QGVAHTTGGA DQRLVVDIGG ASTELVTGTG AQTTSLFSLS MGCVTWLERY FSDRNLAQEN FDDAEKAARD VLRPVADELR FHGWKVCVGA SGTVQALQEI MMAQGMDERI TLAKLQQLKQ RAIQCGRLEE LEIEGLTLER ALVFPSGLAI LIAIFTELNI QSMTLAGGAL REGLVYGMLH LAVDQDIRSR TLRNIQRRFI VDTDQANRVA KLADNFLKQV ENAWHIEPIS RELLLSACQL HEIGLSVDFK QAPYHAAYLV RHLDLPGYTP AQKKLLATLL LNQTNPVDLS SLHQQNAVPP RVAEQLCRLL RLAILFAGRR RDDLVPEITL QALNENLTLT LPSDWLAHHP LGKELIDQES QWQSYVHWPL DVR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Catalyzes the conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.
Protein Families GppA/Ppx family, GppA subfamily
Database Links

KEGG: spq:SPAB_04859

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