Recombinant Salmonella typhimurium Ethanolamine utilization protein EutG (eutG)

Code CSB-YP336907SXB
MSDS
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Source Yeast
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Code CSB-EP336907SXB
MSDS
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Source E.coli
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Code CSB-EP336907SXB-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336907SXB
MSDS
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Source Baculovirus
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Code CSB-MP336907SXB
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
eutG
Uniprot No.
Alternative Names
eutG; STM2461Ethanolamine utilization protein EutG; EC 1.1.-.-
Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Expression Region
1-395
Target Protein Sequence
MQAELQTALF QAFDTLNLQR VKTFSVPPVT LCGLGALGAC GQEAQARGVS HLFVMVDSFL HQAGMTAPLA RSLAMKGVAM TVWPCPPGEP CITDVCAAVA QLREAACDGV VAFGGGSVLD AAKAVALLVT NPDQTLSAMT EHSTLRPRLP LIAVPTTAGT GSETTNVTVI IDAVSGRKQV LAHASLMPDV AILDAAVTEG VPPNVTAMTG IDALTHAIEA YSALNATPFT DSLAIGAIAM IGKSLPKAVG YGHDLAAREN MLLASCMAGM AFSSAGLGLC HAMAHQPGAA LHIPHGQANA MLLPTVMGFN RMVCRERFSQ IGRALTNKKS DDRDAIAAVC ELIAEVGQSK RLADAGAKPE HYSAWAQAAL EDICLRSNPR TATQAQIIDL YAAAG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably acts on the acetaldehyde produced by the degradation of ethanolamine, producing ethanol.; Expression of the eut operon allows this bacteria to use ethanolamine (EA) as a carbon, nitrogen and energy source. It relies on cobalamin (vitamin B12) both as a cofactor for the ethanolamine ammonia-lyase (EAL) activity and to induce the operon. EA enhances bacterial survival in macrophages in a concentration-dependent manner, suggesting it is an important nutrient during infection.
Gene References into Functions
  1. The EutG enzyme converts acetaldehyde to ethanol, which may improve carbon retention by forming acetals; alternatively, EutG may recycle NADH within the carboxysome. PMID: 16585748
Subcellular Location
Bacterial microcompartment.
Protein Families
Iron-containing alcohol dehydrogenase family
Database Links

KEGG: stm:STM2461

STRING: 99287.STM2461

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