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Recombinant Salmonella typhimurium Magnesium-transporting ATPase, P-type 1 (mgtA), partial

Recombinant Salmonella typhimurium Magnesium-transporting ATPase, P-type 1 (mgtA), partial

Code	CSB-YP334160SXB
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP334160SXB
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP334160SXB-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP334160SXB
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP334160SXB
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

mgtA

Uniprot No.

P36640

Alternative Names

mgtA; STM4456Magnesium-transporting ATPase; P-type 1; EC 7.2.2.14; Mg(2+) transport ATPase; P-type 1

Species

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Mediates magnesium influx to the cytosol.

Gene References into Functions

Proline codons present an impediment to the translation of mgtL, which can be alleviated by high [Mg(2+)] exerted on component(s) of the translation machinery, such as EF-P, TrmD, or a ribosomal factor. Inadequate [Mg(2+)] precludes this alleviation, making mgtL translation inefficient and thereby permitting mgtA transcription. PMID: 27849575
We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg(2+). PMID: 24778260
The leader RNA of the Mg(2+) transporter gene mgtA of Salmonella enterica, known to function as a Mg(2+)-sensing riboswitch, harbors an 18 codon proline-rich open reading frame-termed mgtL-that permits intracellular proline to regulate mgtA expression. PMID: 20813261
The PhoP/PhoQ system governs mgtA transcription initiation at all Mg(2+) concentrations and that the Mg(2+)-sensing 5' leader region of the mgtA gene controls transcription elongation into the mgtA coding region when grown in media with <50 muM Mg(2+). PMID: 19897653
The paper shows that Rob is able to induce mgtA transcription in a PhoP-independent fashion by binding to a conserved Mar/Sox/Rob motif localized downstream of the PhoP-box and overlapping the PhoP-dependent transcriptional start site. PMID: 18487336
The PhoP/PhoQ system promotes the expression of the Mg(2+) transporter mgtA gene only when activated in bacteria starved for Mg(2+). PMID: 19801407