Recombinant Salmonella typhimurium Putative transcriptional regulatory protein ptsJ (ptsJ)

Code CSB-YP336691SXB
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Source Yeast
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Code CSB-EP336691SXB
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Source E.coli
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Code CSB-EP336691SXB-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336691SXB
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Source Baculovirus
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Code CSB-MP336691SXB
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ptsJ
Uniprot No.
Alternative Names
ptsJ; STM2436; Vitamin B6 salvage pathway transcriptional repressor PtsJ; HTH-type transcriptional regulatory protein PtsJ
Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Expression Region
1-430
Target Protein Sequence
MIDGKTANEI FDSIRQHIIA GTLRAEDSLP PVRELASELK VNRNTVAAAY KRLITAGLAQ SLGRNGTVIK GSPSPVALEG GDPHTPLHDL SGGNPDPQRL PDLSRYFARL SRTPHLYGDA PVSPELHAWA ARWLRDATPV AGEIDITSGA IDAIERLLCA HLLPGDSVAV EDPCFLSSIN MLRYAGFSAS PVSVDSEGMQ PEKLERALNQ GARAVILTPR AHNPTGCSLS ARRAAALQNM LARYPQVVVI IDDHFALLSS SPWQPVIAQT TQHWAVIRSV SKTLGPDLRL AIVASDSATS AKLRLRLNAG SQWVSHLLQD LVYACLTDPE YQHRLTQTRL FYAARQQKLA RALQQYGIAI SPGDGVNAWL PLDTHSQATA FTLAKSGWLV REGEAFGVSA PSHGLRITLS TLNDSEINTL AADIHQALNR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as transcriptional repressor of the pdxK gene, encoding a pyridoxal kinase involved in the vitamin B6 salvage pathway. Also represses transcription of its own gene. Binds to the ptsJ-pdxK intergenic region, but does not bind pdxY and pdxH promoters. Among all six B6 vitamers, only pyridoxal 5'-phosphate (PLP) clearly binds to the protein and acts as an effector molecule for PtsJ, inducing a protein conformational change that increases affinity for DNA. Thus, PLP stabilizes protein-DNA interactions, reinforcing repression.
Gene References into Functions
  1. PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway. PMID: 27987384
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: stm:STM2436

STRING: 99287.STM2436

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